Metabonomics Combined with UPLC-MS Chemical Profile for Discovery of Antidepressant Ingredients of a Traditional Chinese Medicines Formula, Chaihu-Shu-Gan-San.
Metabonomics Combined with UPLC-MS Chemical Profile for Discovery of Antidepressant Ingredients of a Traditional Chinese Medicines Formula, Chaihu-Shu-Gan-San.
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代谢组学结合 UPLC-MS 化学特征发现中药柴胡舒肝散的抗抑郁成分
DOI：
10.1155/2013/487158
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Zou Z
中科院分区：
文献类型：
--
作者：
Jia H;Su Z;Long W;Liu Y;Chang X;Zhang H;Ding G;Feng Y;Cai D;Zou Z
本研究为中药复方柴胡疏肝散的活性成分研究提供了新的思路。本研究将代谢组学和化学谱相结合，结合多变量统计分析（MVA），寻找CSGS抗抑郁作用的化学成分。根据CSGS与QZ（CSGS without Zhi-Qiao）提取物化学成分的差异，以及CSGS对CSGS引起的代谢紊乱的调节作用，认为辛内酯、柚皮苷、橙皮苷和新橙皮苷是从全方中减去“栀乔”后CSGS的活性成分。它们参与了偏离代谢物2-4、10-14和22-25的调节，涉及酮体合成、苯丙氨酸、酪氨酸和色氨酸生物合成、缬氨酸、天冬氨酸、谷氨酸代谢和糖酵解/糖异生的代谢途径。此外，单胺氧化酶-A活性测定证实了柚皮苷的潜在抗抑郁作用，并通过分子对接研究推测了其在单胺氧化酶-A上的活性位点。将代谢组学与化学谱学相结合，可以揭示中药方剂中的有效化学成分及其对方剂功效的贡献。
This study proposed a new strategy for uncovering the active chemical constituents of a traditional Chinese medicines (TCMs) formula, Chaihu-Shu-Gan-San (CSGS). Metabonomics and chemical profile were integrated in combination with the multivariate statistical analysis (MVA) to discover the chemical constituents which contribute to the antidepressant effect of CSGS. Based upon the difference between CSGS and QZ (CSGS without Zhi-Qiao) extracts in the chemical profiles and the regulations of metabolic disturbances induced by CUMS, synephrine, naringin, hesperidin, and neohesperidin were recognized as the active constituents of CSGS from Zhi-qiao responsible for those missing regulations of CSGS when Zhi-Qiao was subtracted from the whole formula. They participated in the regulations of the deviated metabolites 2–4, 10–14, and 22–25, involved in metabolic pathways of ketone bodies synthesis, phenylalanine, tyrosine and tryptophan biosynthesis, valine, aspartate, glutamate metabolism, and glycolysis/gluconeogenesis. Furthermore, the assay of MAO-A activity confirmed the potential antidepressant effect of naringin and its active sites on the MAO-A was inferred by molecular docking study. The integration of metabonomics and chemical profile was proved to be a useful strategy for uncovering what the active chemical constituents in TCM formula are and how they make contributions for the efficacy of the formula.
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发表时间：
2011-06-01
影响因子：
3.4
作者：
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