Regulation of the S100 protein and GFAP genes is mediated by two common mechanisms in RT4 neuro-glial cell lines.
Regulation of the S100 protein and GFAP genes is mediated by two common mechanisms in RT4 neuro-glial cell lines.
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S100 蛋白和 GFAP 基因的调节由 RT4 神经胶质细胞系中的两种常见机制介导。
DOI：
10.1016/0014-4827(89)90242-5
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发表时间：
1989
影响因子：
3.7
通讯作者：
Sueoka,N
中科院分区：
文献类型：
--
作者：
Freeman,MR;Beckmann,SL;Sueoka,N
RT4-AC细胞表达神经元和神经胶质性质，并在培养中经历细胞类型转化为三种不同的衍生物，描述为神经元样或神经胶质样。RT4-AC细胞中胶质细胞酸性蛋白（GFAP）和S100蛋白表达的协同诱导响应于高细胞密度或细胞内环AMP水平升高而发生。RNA杂交结果表明cAMP协同诱导S100蛋白和GFAP基因表达。此外，我们第一次提供了直接的证据表明，表达S100和GFAP基因的能力是保守的细胞类型转换为神经胶质衍生细胞类型，但协调失去转换为神经元衍生细胞类型。这些结果使得GFAP和S100基因很可能在RT4-AC细胞中由两种常见机制调节：（1）cAMP介导的基因表达控制;和（2）允许这两个基因作为细胞类型转换的结果而协调表达或不表达的机制。
RT4-AC cells express both neuronal and glial properties and undergo cell-type conversion in culture to three distinct derivatives, described as either neuronal-like or glial-like. A coordinate induction of glial fibrillary acidic protein (GFAP) and S100 protein expression in RT4-AC cells occurs in response to either high cell density or elevated levels of intracellular cyclic AMP. RNA hybridization data indicate that S100 protein and GFAP gene expression is coordinately induced by cAMP. In addition, for the first time we provide direct evidence that the ability to express both the S100 and GFAP genes is conserved with cell-type conversion to the glial derivative cell types, but is coordinately lost with conversion to the neuronal derivative cell types. These results make it highly likely that the GFAP and S100 genes are regulated by two common mechanisms in RT4-AC cells: (1) cAMP-mediated control of gene expression; and (2) a mechanism that allows these two genes to be coordinately expressed or not expressed as a consequence of cell-type conversion.
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