Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus.
Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus.
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DOI：
10.1007/s00705-012-1543-0
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发表时间：
2013-04
影响因子：
2.7
通讯作者：
Schmidt MF
中科院分区：
文献类型：
--
作者：
Chai W;Burwinkel M;Wang Z;Palissa C;Esch B;Twardziok S;Rieger J;Wrede P;Schmidt MF
肠道病原性冠状病毒传染性胃肠炎病毒（TGEV）可引起仔猪严重疾病。我们研究了益生菌屎肠球菌NCIMB 10415（E. faecium），其在欧盟被批准作为饲料添加剂，以对抗TGEV感染。E.在TGEV感染之前、同时或之后将屎加入猪睾丸（ST）细胞中。活力测定表明，E.屎肠球菌导致TGEV感染的细胞的存活力的剂量依赖性拯救，几乎达到完全保护。E.粪便处理的培养物减少了高达3个log 10单位。纯化的TGEV蛋白质印迹分析显示，E.粪便处理用透射电镜观察了TGEV颗粒在E.这可能是一种捕获病毒和防止感染的手段。E.屎和白细胞介素6和8的表达升高指出，刺激细胞防御作为一种机制，以打击TGEV感染。
The enteropathogenic coronavirus transmissible gastroenteritis virus (TGEV) causes severe disease in young piglets. We have studied the protective effects of the probiotic Enterococcus faecium NCIMB 10415 (E. faecium), which is approved as a feed additive in the European Union, against TGEV infection. E. faecium was added to swine testicle (ST) cells before, concomitantly with, or after TGEV infection. Viability assays revealed that E. faecium led to a dose-dependent rescue of viability of TGEV-infected cells reaching nearly to complete protection. Virus yields of the E. faecium–treated cultures were reduced by up to three log10 units. Western blot analysis of purified TGEV revealed that the levels of all viral structural proteins were reduced after E. faecium treatment. Using transmission electron microscopy, we observed attachment of TGEV particles to the surface of E. faecium which might be a means to trap virus and to prevent infection. Increased production of nitric oxide in the cells treated with E. faecium and elevated expression of interleukin 6 and 8 pointed to stimulated cellular defense as a mechanism to fight TGEV infection.
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DOI：
10.1073/pnas.0910307107
发表时间：
2010-01-05
影响因子：
11.1
作者：
Pagnini, Cristiano;Saeed, Rubina;Cominelli, Fabio
通讯作者：
Cominelli, Fabio
影响因子：
2.2
作者：
Liu, Fangning;Li, Guohua;Yuan, Lijuan
通讯作者：
Yuan, Lijuan
影响因子：
9.8
作者：
Lammers, KM;Helwig, U;Campieri, M
通讯作者：
Campieri, M
影响因子：
1.8
作者：
Jung K;Gurnani A;Renukaradhya GJ;Saif LJ
通讯作者：
Saif LJ
影响因子：
2.7
作者：
Freitas, M;Tavan, E;Trugnan, G
通讯作者：
Trugnan, G