Nitric oxide is elicited and inhibits viral replication in pigs infected with porcine respiratory coronavirus but not porcine reproductive and respiratory syndrome virus.
Nitric oxide is elicited and inhibits viral replication in pigs infected with porcine respiratory coronavirus but not porcine reproductive and respiratory syndrome virus.
复制标题
DOI：
10.1016/j.vetimm.2010.03.022
复制
发表时间：
2010-08-15
影响因子：
1.8
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Jung K;Gurnani A;Renukaradhya GJ;Saif LJ
关于一氧化氮（·NO）在呼吸道冠状病毒（CoV）感染的先天免疫中的作用的信息很少。我们通过Greiss试验检测了感染猪呼吸道冠状病毒（PRCV）或猪繁殖与呼吸综合征病毒（PRRSV）（一种Nidovirales成员，如CoV）的猪支气管肺泡灌洗液（BAL）中的·NO水平。用一氧化氮供体S-亚硝基-N-乙酰青霉胺（SNAP）体外抗病毒实验，观察了一氧化氮对这两种病毒的抗病毒作用。我们检测到PRCV感染猪的BAL液中·NO水平大幅增加，但在PRRSV感染猪中则没有。PRCV诱导的肺上皮细胞坏死与·NO升高相一致。此外，PRRSV感染的肺泡巨噬细胞（AM）细胞培养基中的·NO水平与模拟感染的AM无差异。抗病毒试验表明，·NO显著抑制PRCV在猪睾丸（ST）细胞中的复制，而PRRSV对·NO不敏感。我们的研究表明，与在AM中诱导细胞凋亡的PRRSV不同，呼吸道CoV如PRCV感染肺上皮细胞并引起细胞溶解，诱导呼吸道中产生·NO。因此，·NO可能通过抑制病毒复制在呼吸道CoV感染的先天免疫中发挥作用。
There is little information on the role of nitric oxide (•NO) in innate immunity to respiratory coronavirus (CoV) infections. We examined •NO levels by Greiss assay in bronchoalveolar lavage (BAL) of pigs infected with either porcine respiratory coronavirus (PRCV) or porcine reproductive and respiratory syndrome virus (PRRSV), a member of Nidovirales, like CoV. The antiviral effects of •NO on these two viruses were tested in an in vitro system using a •NO donor, S-nitroso-N-acetylpenicillamine (SNAP). We detected a large increase in •NO levels in BAL fluids of PRCV-infected pigs, but not in PRRSV-infected pigs. Pulmonary epithelial cell necrosis induced by PRCV coincided with increased •NO. Moreover, •NO levels in cell culture medium of PRRSV-infected alveolar macrophages (AMs) did not differ from that of mock-infected AMs. Antiviral assays showed that •NO significantly inhibited PRCV replication in swine testicular (ST) cells, whereas PRRSV was not susceptible to •NO based on the conditions tested. Our study suggests that unlike PRRSV which induces apoptosis in AMs, respiratory CoVs such as PRCV that infect pulmonary epithelial cells and cause cytolysis, induce •NO production in the respiratory tract. Thus, •NO may play a role in innate immunity to respiratory CoV infections by inhibiting viral replication.
登录
查看更多内容
影响因子：
2.8
作者：
Schroder, Kate;Sweet, Matthew J.;Hume, David A.
通讯作者：
Hume, David A.
影响因子：
2.4
作者：
COX, E;HOOYBERGHS, J;PENSAERT, MB
通讯作者：
PENSAERT, MB
DOI：
10.1111/j.1439-0450.2004.00732.x
发表时间：
2004-03-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Jung, K;Ha, Y;Chae, C
通讯作者：
Chae, C
影响因子：
3.8
作者：
Jung, Kwonil;Renukaradhya, Gourapura J.;Saif, Linda J.
通讯作者：
Saif, Linda J.
影响因子：
5.4
作者：
Åkerström, S;Mousavi-Jazi, M;Mirazimi, A
通讯作者：
Mirazimi, A