Specialized lysophosphatidic acid acyltransferases contribute to unusual fatty acid accumulation in exotic Euphorbiaceae seed oils
Specialized lysophosphatidic acid acyltransferases contribute to unusual fatty acid accumulation in exotic Euphorbiaceae seed oils
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特殊的溶血磷脂酸酰基转移酶有助于外来大戟科种子油中异常的脂肪酸积累
DOI：
10.1007/s00425-018-03086-y
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发表时间：
2019
期刊：
影响因子：
4.3
通讯作者：
Bates, Philip D.
中科院分区：
文献类型：
--
作者：
Shockey, Jay;Lager, Ida;Stymne, Sten;Kotapati, Hari Kiran;Sheffield, Jennifer;Mason, Catherine;Bates, Philip D.
主要结论大戟科植物三酰甘油组装酶底物选择性的体内和体外分析与种子特异性不寻常脂肪酸产生的协同进化是一致的，并且表明这些基因中的许多将用于生物技术生产设计师油。（Vernicia fordii）、蓖麻子（Ricinus communis）、Bernardia pulchella和Euphorbia lagascae在其种子油中积累了不寻常的脂肪酸，其中许多具有化学工业的价值。然而，各种不利的植物特性，包括低种子产量，有毒化合物的产生，有限的生长范围，以及对非生物胁迫的抗性差，限制了这些植物的充分的农艺开发。利用生物技术在高产非食用油料作物中生产这些不寻常的脂肪酸（乌法）将为这些有价值的生物化学品提供新的可靠来源。以前的研究表明，主要的乌法生物合成基因的表达单独是不够的高水平的积累在转基因种子油，其他基因必须包括驱动选择性乌法掺入油。在这里，我们使用了一系列的植物分子遗传学研究和体外生物化学测量，以证明溶血磷脂酸酰基转移酶从twoEuphorbiaceaespecies具有高选择性，其各自的不寻常的脂肪酸纳入磷脂酸中间体的石油生物合成。这些结果是一致的假设，即不寻常的脂肪酸积累产生的部分通过多种油的生物合成和组装酶，合作，以提高选择性的脂肪酸掺入到种子油中的膜脂中发现的常见脂肪酸的共同进化。
Main conclusionIn vivo and in vitro analyses ofEuphorbiaceaespecies’ triacylglycerol assembly enzymes substrate selectivity are consistent with the co-evolution of seed-specific unusual fatty acid production and suggest that many of these genes will be useful for biotechnological production of designer oils.Many exoticEuphorbiaceaespecies, including tung tree (Vernicia fordii), castor bean (Ricinus communis),Bernardia pulchella, andEuphorbia lagascae, accumulate unusual fatty acids in their seed oils, many of which have valuable properties for the chemical industry. However, various adverse plant characteristics including low seed yields, production of toxic compounds, limited growth range, and poor resistance to abiotic stresses have limited full agronomic exploitation of these plants. Biotechnological production of these unusual fatty acids (UFA) in high yielding non-food oil crops would provide new robust sources for these valuable bio-chemicals. Previous research has shown that expression of the primary UFA biosynthetic gene alone is not enough for high-level accumulation in transgenic seed oils; other genes must be included to drive selective UFA incorporation into oils. Here, we use a series of in planta molecular genetic studies and in vitro biochemical measurements to demonstrate that lysophosphatidic acid acyltransferases from twoEuphorbiaceaespecies have high selectivity for incorporation of their respective unusual fatty acids into the phosphatidic acid intermediate of oil biosynthesis. These results are consistent with the hypothesis that unusual fatty acid accumulation arose in part via co-evolution of multiple oil biosynthesis and assembly enzymes that cooperate to enhance selective fatty acid incorporation into seed oils over that of the common fatty acids found in membrane lipids.
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影响因子：
13.8
作者：
Burgal J;Shockey J;Lu C;Dyer J;Larson T;Graham I;Browse J
通讯作者：
Browse J
DOI：
10.1016/j.jchromb.2018.10.012
发表时间：
2018-12
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
H. K. Kotapati;Philip D. Bates
通讯作者：
H. K. Kotapati;Philip D. Bates
三氟乙酸酐催化甘油磷酸胆碱的轻松酰化。
影响因子：
6.5
作者：
P. Kanda;M. Wells
通讯作者：
M. Wells
蓖麻 (Ricinus communis L.)
影响因子：
4
作者：
T. Mckeon
通讯作者：
T. Mckeon
影响因子：
2.9
作者：
HARA, A;RADIN, NS
通讯作者：
RADIN, NS