Enteric virome negatively affects seroconversion following oral rotavirus vaccination in a longitudinally sampled cohort of Ghanaian infants.
Enteric virome negatively affects seroconversion following oral rotavirus vaccination in a longitudinally sampled cohort of Ghanaian infants.
复制标题
DOI：
10.1016/j.chom.2021.12.002
复制
发表时间：
2022-01-12
影响因子：
30.3
通讯作者：
Harris VC
中科院分区：
文献类型：
--
作者：
Kim AH;Armah G;Dennis F;Wang L;Rodgers R;Droit L;Baldridge MT;Handley SA;Harris VC
轮状病毒疫苗（RVVs）大大降低了严重轮状病毒胃肠炎的死亡率，但在低收入和中等收入国家（LMICs）的效果明显较差，限制了其挽救生命的潜力。RVV有效性降低的病因尚不完全清楚，但肠道微生物群与调节对RVV的免疫有关。在这里，我们分析了122名加纳婴儿的纵向队列肠道微生物群，在6至15周龄的3次Rotarix疫苗接种过程中进行评估，以评估细菌和病毒种群在非血清转化和血清转化婴儿之间是否存在差异。我们鉴定了细菌分类群，包括链球菌和肠杆菌科中分类较差的分类群，它们与血清转化呈正相关。相比之下，噬菌体多样性以及肠道病毒B和多种新型辅酶病毒的检测与RVV血清转化呈负相关。这些发现表明，病毒组-裂谷病毒干扰是中低收入国家疫苗性能差的一个未被充分认识的原因。接种轮状病毒疫苗的加纳婴儿微生物群的纵向分析链球菌和肠杆菌科类群与RVV血清转化呈正相关肠道病毒B、cosav病毒A和噬菌体丰富度与RVV血清状态负相关轮状病毒疫苗在低收入和中等收入国家的效果下降，使儿童易患危及生命的胃肠炎。Kim等人对122名加纳婴儿纵向收集的粪便样本进行了无偏倚宏基因组测序，发现病毒组成员与轮状病毒疫苗免疫原性受限显著相关，这可能解释了这种性能下降的原因。
Rotavirus vaccines (RVVs) have substantially diminished mortality from severe rotavirus (RV) gastroenteritis but are significantly less effective in low- and middle-income countries (LMICs), limiting their life-saving potential. The etiology of RVV’s diminished effectiveness remains incompletely understood, but the enteric microbiota has been implicated in modulating immunity to RVVs. Here, we analyze the enteric microbiota in a longitudinal cohort of 122 Ghanaian infants, evaluated over the course of 3 Rotarix vaccinations between 6 and 15 weeks of age, to assess whether bacterial and viral populations are distinct between non-seroconverted and seroconverted infants. We identify bacterial taxa including Streptococcus and a poorly classified taxon in Enterobacteriaceae as positively correlating with seroconversion. In contrast, both bacteriophage diversity and detection of Enterovirus B and multiple novel cosaviruses are negatively associated with RVV seroconversion. These findings suggest that virome-RVV interference is an underappreciated cause of poor vaccine performance in LMICs. Longitudinal analysis of microbiota of Ghanaian infants receiving rotavirus vaccine Streptococcus and Enterobacteriaceae taxa positively associate with RVV seroconversion Enterovirus B, Cosavirus A, and phage richness negatively associate with RVV serostatus Rotavirus vaccines have diminished performance in low- and middle-income countries, leaving children vulnerable to life-threatening gastroenteritis. Kim et al. employed unbiased metagenomic sequencing of longitudinally collected fecal samples from 122 Ghanaian infants and found that members of the virome were significantly associated with constrained rotavirus vaccine immunogenicity, potentially explaining this diminished performance.
登录
查看更多内容
影响因子：
4.8
作者：
Finkbeiner SR;Holtz LR;Jiang Y;Rajendran P;Franz CJ;Zhao G;Kang G;Wang D
通讯作者：
Wang D
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.3
作者：
Fix, Jonathan;Chandrashekhar, Kshipra;Becker-Dreps, Sylvia
通讯作者：
Becker-Dreps, Sylvia
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
10.7
作者：
Gascuel, O
通讯作者：
Gascuel, O