Local Increases in Mechanical Tension Shape Compartment Boundaries by Biasing Cell Intercalations
Local Increases in Mechanical Tension Shape Compartment Boundaries by Biasing Cell Intercalations
复制标题
通过偏置细胞插层局部增加机械张力形状室边界
DOI：
10.1016/j.cub.2014.06.052
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发表时间：
2014
期刊：
影响因子：
9.2
通讯作者：
Dahmann
中科院分区：
文献类型：
--
作者：
Umetsu;Aigouy;Jülicher;Dahmann
机械力在发育中的动物的组织形成过程中起着重要的作用。许多组织被组织成相邻的、非混合的细胞群，称为隔室[1-7]。隔室之间的边界显示出笔直的形态，并且与对组织生长和图案化重要的信号中心相关[8]。最近已经证明，沿着隔室边界的细胞连接处的机械张力的局部增加可以防止细胞混合并保持直线边界[9-13]。然而，局部机械张力增加阻止细胞在室边界混合的细胞机制仍然知之甚少。在这里，我们已经使用了实时成像和定量图像分析，以确定细胞动力学前后室边界附近的theDrosophilapupal腹部表皮。我们表明，细胞混合室内涉及多个细胞嵌入。细胞嵌入的频率和方向是不变的沿着的隔室边界，而是，在嵌入过程中的交界处的收缩的不对称性偏置，导致抑制细胞混合的细胞重排。组织生长的模拟表明，机械张力的局部增加可以解释连接收缩的这种偏差。我们的结论是，局部增加机械张力保持细胞群体分开的影响交界处的重排过程中细胞嵌入。
Mechanical forces play important roles during tissue organization in developing animals. Many tissues are organized into adjacent, nonmixing groups of cells termed compartments [1–7]. Boundaries between compartments display a straight morphology and are associated with signaling centers that are important for tissue growth and patterning [8]. Local increases in mechanical tension at cell junctions along compartment boundaries have recently been shown to prevent cell mixing and to maintain straight boundaries [9–13]. The cellular mechanisms by which local increases in mechanical tension prevent cell mixing at compartment boundaries, however, remain poorly understood. Here, we have used live imaging and quantitative image analysis to determine cellular dynamics at and near the anteroposterior compartment boundaries of theDrosophilapupal abdominal epidermis. We show that cell mixing within compartments involves multiple cell intercalations. Frequency and orientation of cell intercalations are unchanged along the compartment boundaries; rather, an asymmetry in the shrinkage of junctions during intercalation is biased, resulting in cell rearrangements that suppress cell mixing. Simulations of tissue growth show that local increases in mechanical tension can account for this bias in junctional shrinkage. We conclude that local increases in mechanical tension maintain cell populations separate by influencing junctional rearrangements during cell intercalation.
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