Evaluating the impact of sample storage, handling, and technical ability on the decay and recovery of SARS-CoV-2 in wastewater.
Evaluating the impact of sample storage, handling, and technical ability on the decay and recovery of SARS-CoV-2 in wastewater.
复制标题
DOI：
10.1371/journal.pone.0270659
复制
发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Noble, Rachel T.
中科院分区：
文献类型：
--
作者：
Beattie, Rachelle E.;Blackwood, A. Denene;Clerkin, Thomas;Dinga, Carly;Noble, Rachel T.
污水流行病学有助于跟踪和监测社区的疾病流行水平，并已被广泛用于补充当前新冠肺炎大流行期间的临床检测。尽管有许多好处，但样品存储、处理和处理方法中的可变性来源可能会使WBE数据难以推广。我们进行了一项实验，以确定WBE数据的变异性来源，包括存储时间、处理和处理技术对北卡罗来纳州三个污水处理厂(WWTP)19天废水进水中SARS-CoV-2浓度的影响。在19天的实验中，保持在4°C的进水样中的SARS-CoV-2浓度没有显著下降。加热巴氏杀菌对三个污水处理厂中的两个的SARS-CoV-2浓度没有显著影响，但确实降低了人口规模最小的污水处理厂的病毒回收率。在每个处理日期，立即处理每个样本中的一个过滤器，同时将复制过滤器冻结在-80°C。处理后，发现先前冷冻的过滤器的浓度(<；0.2个对数拷贝/L)略高于立即处理的对应过滤器，这表明冷冻过滤器是一种可行的延迟量化方法，甚至可能提高低病毒浓度污水处理厂的回收率。对样品处理过程中影响变异性的因素的调查表明，分析员的经验水平对可接受的液滴产生有显著的贡献(p<；0.001)，而提取效率和反转录效率对SARS-CoV-2的日常变异性有显著的贡献(p<；0.05)。这项研究为最大限度地减少废水流入中SARS CoV-2的腐烂和/或损失，同时遵守安全程序，促进有效的实验室工作流程，并考虑变异性的来源提供了有价值的实用信息。
Wastewater based epidemiology (WBE) is useful for tracking and monitoring the level of disease prevalence in a community and has been used extensively to complement clinical testing during the current COVID-19 pandemic. Despite the numerous benefits, sources of variability in sample storage, handling, and processing methods can make WBE data difficult to generalize. We performed an experiment to determine sources of variability in WBE data including the impact of storage time, handling, and processing techniques on the concentration of SARS-CoV-2 in wastewater influent from three wastewater treatment plants (WWTP) in North Carolina over 19 days. The SARS-CoV-2 concentration in influent samples held at 4°C did not degrade significantly over the 19-day experiment. Heat pasteurization did not significantly impact the concentration of SARS-CoV-2 at two of the three WWTP but did reduce viral recovery at the WWTP with the smallest population size served. On each processing date, one filter from each sample was processed immediately while a replicate filter was frozen at -80°C. Once processed, filters previously frozen were found to contain slightly higher concentrations (<0.2 log copies/L) than their immediately processed counterparts, indicating freezing filters is a viable method for delayed quantification and may even improve recovery at WWTP with low viral concentrations. Investigation of factors contributing to variability during sample processing indicated that analyst experience level contributed significantly (p<0.001) to accepted droplet generation while extraction efficiency and reverse transcription efficiency contributed significantly (p<0.05) to day-to-day SARS-CoV-2 variability. This study provides valuable practical information for minimizing decay and/or loss of SARS CoV-2 in wastewater influent while adhering to safety procedures, promoting efficient laboratory workflows, and accounting for sources of variability.
登录
查看更多内容
DOI：
10.1016/j.scitotenv.2021.145732
发表时间：
2021-06-20
期刊：
The Science of the total environment
影响因子：
--
作者：
Brisolara KF;Maal-Bared R;Sobsey MD;Reimers RS;Rubin A;Bastian RK;Gerba C;Smith JE;Bibby K;Kester G;Brown S
通讯作者：
Brown S
影响因子：
4.6
作者：
Dobnik D;Štebih D;Blejec A;Morisset D;Žel J
通讯作者：
Žel J
影响因子：
5.1
作者：
Cholet, Fabien;Ijaz, Umer Z.;Smith, Cindy J.
通讯作者：
Smith, Cindy J.
DOI：
10.1021/acsestwater.1c00160
发表时间：
2021-07-09
期刊：
ACS ES&T WATER
影响因子：
--
作者：
Feng, Shuchen;Roguet, Adelaide;McLellan, Sandra L.
通讯作者：
McLellan, Sandra L.
影响因子：
9.3
作者：
Huggett, Jim F.;Foy, Carole A.;Bustin, Stephen A.
通讯作者：
Bustin, Stephen A.