Reticulocytes in donor blood units enhance red blood cell alloimmunization.
Reticulocytes in donor blood units enhance red blood cell alloimmunization.
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DOI：
10.3324/haematol.2023.282815
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发表时间：
2023-10-01
期刊：
影响因子：
10.1
通讯作者：
Hudson, Krystalyn E.
中科院分区：
文献类型：
--
作者：
Thomas, Tiffany A.;Qiu, Annie;Kim, Christopher Y.;Gordy, Dominique E.;Miller, Anabel;Tredicine, Maria;Dzieciatkowska, Monika;Dei Zotti, Flavia;Hod, Eldad A.;D'Alessandro, Angelo;Zimring, James C.;Spitalnik, Steven L.;Hudson, Krystalyn E.
虽然红细胞（RBC）输注可以挽救生命，但一些患者会产生针对供体血型抗原的具有临床意义的同种异体抗体，从而在多种临床环境中产生不良影响。几乎没有有效的措施可以预防RBC同种免疫和/或消除致敏患者的同种抗体。供体相关因素可能会影响同种异体免疫;因此，有一个未满足的临床需要，以确定哪些红细胞单位是免疫原性的。与健康的非献血者相比，重复志愿献血者和服用铁补充剂的献血者的网织红细胞计数升高。早期网织红细胞保留线粒体和其他成分，这可能是免疫反应中的危险信号。在此，我们测试了供体RBC单位中的网织红细胞是否可以增强RBC同种异体免疫。使用小鼠模型，我们证明，使用供体RBC单位与增加网织红细胞频率的剂量依赖性增加RBC同种免疫率和同种抗体水平。输注富含网织红细胞的RBC单位与循环中RBC清除率增加和强促炎细胞因子反应相关。与先前报告的输血后RBC消耗模式相比，脾B细胞越来越多地进行网织红细胞富集单位的红细胞吞噬作用。这些数据表明，捐献的RBC单位中的网织红细胞会影响输血的质量，靶向不同的隔室，并且可能是RBC同种异体免疫的一个未被充分认识的风险因素。
Although red blood cell (RBC) transfusions save lives, some patients develop clinically-significant alloantibodies against donor blood group antigens, which then have adverse effects in multiple clinical settings. Few effective measures exist to prevent RBC alloimmunization and/or eliminate alloantibodies in sensitized patients. Donor-related factors may influence alloimmunization; thus, there is an unmet clinical need to identify which RBC units are immunogenic. Repeat volunteer blood donors and donors on iron supplements have elevated reticulocyte counts compared to healthy non-donors. Early reticulocytes retain mitochondria and other components, which may act as danger signals in immune responses. Herein, we tested whether reticulocytes in donor RBC units could enhance RBC alloimmunization. Using a murine model, we demonstrate that transfusing donor RBC units with increased reticulocyte frequencies dose-dependently increased RBC alloimmunization rates and alloantibody levels. Transfusing reticulocyte-rich RBC units was associated with increased RBC clearance from the circulation and a robust proinflammatory cytokine response. As compared to previously reported post-transfusion RBC consumption patterns, erythrophagocytosis from reticulocyte-rich units was increasingly performed by splenic B cells. These data suggest that reticulocytes in a donated RBC unit impact the quality of blood transfused, are targeted to a distinct compartment, and may be an underappreciated risk factor for RBC alloimmunization.
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影响因子：
64.5
作者：
Caielli S;Cardenas J;de Jesus AA;Baisch J;Walters L;Blanck JP;Balasubramanian P;Stagnar C;Ohouo M;Hong S;Nassi L;Stewart K;Fuller J;Gu J;Banchereau JF;Wright T;Goldbach-Mansky R;Pascual V
通讯作者：
Pascual V
DOI：
10.1038/newbio236157a0
发表时间：
1972-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
COME, SE;ROBINSON, SH;SHOHET, SB
通讯作者：
SHOHET, SB
影响因子：
10.1
作者：
Pellegrin S;Severn CE;Toye AM
通讯作者：
Toye AM
影响因子：
8.7
作者：
Baumgarth N
通讯作者：
Baumgarth N
影响因子：
7.3
作者：
Lee JY;Madany E;El Kadi N;Pandya S;Ng K;Yamashita M;Jefferies CA;Gibb DR
通讯作者：
Gibb DR