Assessing protein distribution and dendritic spine morphology relationships using structured illumination microscopy in cultured neurons.
Assessing protein distribution and dendritic spine morphology relationships using structured illumination microscopy in cultured neurons.
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DOI：
10.1016/j.xpro.2023.102829
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发表时间：
2024-03-15
期刊：
影响因子：
--
通讯作者：
Cahill, Michael E.
中科院分区：
文献类型：
--
作者：
Bjornson, Kathryn J.;Cahill, Michael E.
树突棘是树突上的突起，形成大脑内兴奋性连接的突触后方面。树突棘形态与突触功能强度相关，树突棘内蛋白质纳米结构域的空间调节是树突棘结构和功能的重要决定因素。在这里，我们提出了一个协议来解决纳米级的本地化的蛋白质树突棘使用结构照明显微镜。我们描述了树突棘参数的结构分析，蛋白质定位分析和数据处理的步骤。有关本方案使用和执行的完整详细信息，请参见Bjornson et al.通过超分辨率显微镜评估蛋白质在树突棘中的空间定位评估蛋白质在棘形态上的区室化确定蛋白质定位/相互作用对棘结构的影响出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理准则。树突棘是树突上的突起，形成大脑内兴奋性连接的突触后方面。树突棘形态与突触功能强度相关，树突棘内蛋白质纳米结构域的空间调节是树突棘结构和功能的重要决定因素。在这里，我们提出了一个协议来解决纳米级亚区室化的蛋白质树突棘使用结构照明显微镜。我们描述了树突棘参数的结构分析，蛋白质定位分析和数据处理的步骤。
Dendritic spines are protrusions on dendrites forming the postsynaptic aspect of excitatory connections within the brain. Spine morphology is associated with synaptic functional strength and the spatial regulation of protein nanodomains within dendritic spines is an important determinant of spine structure and function. Here, we present a protocol to resolve the nanoscale localization of proteins within dendritic spines using structured illumination microscopy. We describe steps for the structural analysis of dendritic spine parameters, protein localization analysis, and data processing. For complete details on the use and execution of this protocol, please refer to Bjornson et al. Assess protein spatial localization in dendritic spines via super-resolution microscopy Assess the compartmentalization of proteins across spine morphology Determine the effects of protein localization/interaction on spine structure Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Dendritic spines are protrusions on dendrites forming the postsynaptic aspect of excitatory connections within the brain. Spine morphology is associated with synaptic functional strength and the spatial regulation of protein nanodomains within dendritic spines is an important determinant of spine structure and function. Here, we present a protocol to resolve the nanoscale subcompartmentalization of proteins within dendritic spines using structured illumination microscopy. We describe steps for the structural analysis of dendritic spine parameters, protein localization analysis, and data processing.
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