A device for high-throughput monitoring of degradation in soft tissue samples.
A device for high-throughput monitoring of degradation in soft tissue samples.
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一种高通量监测软组织样品降解的装置
DOI：
10.1016/j.jbiomech.2018.04.040
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发表时间：
2018
影响因子：
2.4
通讯作者：
Neidlin M
中科院分区：
文献类型：
--
作者：
Tzeranis DS;Panagiotopoulos IA;Gkouma S;Kanakaris G;Georgiou NA;Vaindirlis N;Neidlin M
这项工作描述了一种新型设备的设计和验证，该设备名为高通量降解监测设备(HDD)，用于监测24个软组织样品在细胞培养孵化器中几天的孵育期内的降解情况。该装置通过监测静态重力载荷引起的样品变形来量化样品的降解。最初的仪器设计和实验方案的制定侧重于量化软骨退变。对主要由热瞬变和仪器传感器平移引起的测量误差的表征表明，硬盘驱动器可以量化样品退化，精度为<；6 μm，温度诱导误差为<；10 μm。在一项初步研究中评估了HDD的能力，该研究监测了胶原酶溶液在三天内对Freshex活体人类软骨样本的降解。HDD可以很好地解决胶原酶浓度低至0.5 mg/ml的影响。仔细的样品制备使测量结果不受供体之间的差异(变异系数为70%)。由于其独特的样品吞吐量、测量精度、时间采样和实验多样性的组合，HDD提供了一个基于生物力学的新型实验平台，用于量化蛋白质(细胞因子、生长因子、酶、抗体)或小分子对软组织或组织工程构建物降解的影响。因此，HDD可以在包括药物筛选和生物材料开发在内的重要应用中补充现有的工具和体外模型。
This work describes the design and validation of a novel device, the High-Throughput Degradation Monitoring Device (HDD), for monitoring the degradation of 24 soft tissue samples over incubation periods of several days inside a cell culture incubator. The device quantifies sample degradation by monitoring its deformation induced by a static gravity load. Initial instrument design and experimental protocol development focused on quantifying cartilage degeneration. Characterization of measurement errors, caused mainly by thermal transients and by translating the instrument sensor, demonstrated that HDD can quantify sample degradation with <6 μm precision and <10 μm temperature-induced errors. HDD capabilities were evaluated in a pilot study that monitored the degradation of freshex vivohuman cartilage samples by collagenase solutions over three days. HDD could robustly resolve the effects of collagenase concentration as small as 0.5 mg/ml. Careful sample preparation resulted in measurements that did not suffer from donor-to-donor variation (coefficient of variance <70%). Due to its unique combination of sample throughput, measurement precision, temporal sampling and experimental versality, HDD provides a novel biomechanics-based experimental platform for quantifying the effects of proteins (cytokines, growth factors, enzymes, antibodies) or small molecules on the degradation of soft tissues or tissue engineering constructs. Thereby, HDD can complement established tools andin vitromodels in important applications including drug screening and biomaterial development.
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具有高通量能力和实时模量测量能力的压缩组织刺激器的设计和验证。
DOI：
10.1089/ten.tec.2011.0233
发表时间：
2012
期刊：
Tissue engineering. Part C, Methods
影响因子：
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作者：
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影响因子：
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作者：
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Leonidas G. Alexopoulos
影响因子：
2.4
作者：
Frank, EH;Jin, M;Grodzinsky, AJ
通讯作者：
Grodzinsky, AJ
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影响因子：
6.9
作者：
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通讯作者：
J. Bateman
影响因子：
3.8
作者：
Aufderheide, Adam C.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.