Comparative bioinformatics analysis between proteomes of rabbit aneurysm model and human intracranial aneurysm with label-free quantitative proteomics.
Comparative bioinformatics analysis between proteomes of rabbit aneurysm model and human intracranial aneurysm with label-free quantitative proteomics.
复制标题
无标记定量蛋白质组学比较兔动脉瘤模型和人颅内动脉瘤的蛋白质组分析
DOI：
10.1111/cns.13570
复制
发表时间：
2021-01
影响因子：
5.5
通讯作者：
Zhu W
中科院分区：
文献类型：
--
作者：
Liu Y;Song Y;Liu P;Li S;Shi Y;Yu G;Quan K;Fan Z;Li P;An Q;Zhu W
本研究旨在通过比较兔颅内动脉瘤模型和人颅内动脉瘤的蛋白质组，发现参与颅内动脉瘤发生发展的关键蛋白。取5例人颅内动脉瘤标本和5例颞浅动脉标本，4例家兔动脉瘤标本和4例对照标本进行蛋白质谱分析。采用4个人颅内动脉瘤样本和4个颞浅动脉样本，6个兔动脉瘤样本和6个对照样本进行免疫化学。蛋白质组学分析显示，人颅内动脉瘤中有180个蛋白显著表达差异，兔颅内动脉瘤中有716个蛋白显著表达差异。其中57个蛋白在两种动物中差异表达，其中24个蛋白在动脉瘤中与对照组相比表达增加，33个蛋白表达减少。蛋白参与了局灶黏附和细胞外基质-受体相互作用途径。我们发现COL4A2、MYLK、VCL和TAGLN可能与动脉瘤的发展有关。蛋白质组学分析为动脉瘤的发病机制提供了基本的见解。与病灶黏附和细胞外基质-受体相互作用途径相关的蛋白质在颅内动脉瘤的发生和发展中起重要作用。利用蛋白质组学分析和细胞模型验证兔和人动脉瘤之间的蛋白表达，通过比较两者之间的蛋白谱，寻找共同基因，并用生物信息学工具进行分析，可以阐明参与颅内动脉瘤发生发展的关键蛋白。
This study aimed to find critical proteins involved in the development of intracranial aneurysm by comparing proteomes of rabbit aneurysm model and human aneurysms. Five human intracranial aneurysm samples and 5 superficial temporal artery samples, and 4 rabbit aneurysm samples and 4 control samples were collected for protein mass spectrometry. Four human intracranial aneurysm samples and 4 superficial temporal artery samples, and 6 rabbit aneurysm samples and 6 control samples were used for immunochemistry. Proteomic analysis revealed 180 significantly differentially expressed proteins in human intracranial aneurysms and 716 significantly differentially expressed proteins in rabbit aneurysms. Among them, 57 proteins were differentially expressed in both species, in which 24 were increased and 33 were decreased in aneurysms compared to the control groups. Proteins were involved in focal adhesion and extracellular matrix‐receptor interaction pathways. We found that COL4A2, MYLK, VCL, and TAGLN may be related to aneurysm development. Proteomics analysis provided fundamental insights into the pathogenesis of aneurysm. Proteins related to focal adhesion and extracellular matrix‐receptor interaction pathways play an important role in the occurrence and development of intracranial aneurysm. Using proteomic analysis and cell model to verify the protein expression between rabbit and human aneurysm, by comparing the protein profiles between these two, looking for common genes and analyzed with bioinformatics tools, can illustrate the critical proteins involves in the development of intracranial aneurysm.
登录
查看更多内容
影响因子：
2.9
作者：
Kadirvel, Ramanathan;Ding, Yong-Hong;Dai, Daying;Lewis, Debra A.;Kallmes, David F.
通讯作者：
Kallmes, David F.
影响因子：
8.3
作者：
Kleinloog, Rachel;Verweij, Bon H.;Ruigrok, Ynte M.
通讯作者：
Ruigrok, Ynte M.
影响因子：
5.5
作者：
Fang, Yuan-Jian;Mei, Shu-Hao;Chen, Sheng
通讯作者：
Chen, Sheng
影响因子：
6.9
作者：
Etminan, Nima;Buchholz, Bruce A.;Dreier, Rita;Bruckner, Peter;Torner, James C.;Steiger, Hans-Jakob;Haenggi, Daniel;Macdonald, R. Loch
通讯作者：
Macdonald, R. Loch
iTRAQ 对破裂和未破裂脑动脉瘤差异表达蛋白的定量蛋白质组学分析
影响因子：
3.3
作者：
Jiang, Pengjun;Wu, Jun;Wang, Shuo
通讯作者：
Wang, Shuo