Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice.
Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice.
复制标题
水稻耐盐性中涉及的新型 Cys2/His2 型锌指蛋白的功能分析
DOI：
10.1093/jxb/erq120
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发表时间：
2010-06
影响因子：
6.9
通讯作者：
Zhang HS
中科院分区：
文献类型：
--
作者：
Sun SJ;Guo SQ;Yang X;Bao YM;Tang HJ;Sun H;Huang J;Zhang HS
Cys2/ his2型锌指蛋白参与植物发育和胁迫反应的不同细胞过程。通过微阵列分析，从水稻幼苗中鉴定并克隆了盐敏感锌指蛋白基因ZFP179。ZFP179编码一个17.95 kDa的蛋白，具有两个具有转录激活活性的c2h2型锌指基序。实时RT-PCR分析表明，ZFP179在未成熟穗中高表达，在NaCl、PEG 6000和ABA处理下均有明显的诱导作用。ZFP179在水稻中的过表达增加了耐盐性，转基因幼苗对外源ABA表现出过敏反应。在盐胁迫下，转基因植株的游离脯氨酸和可溶性糖含量明显高于野生型植株。ZFP179转基因水稻对氧化胁迫的耐受性、清除活性氧（ROS）的能力以及盐胁迫下OsDREB2A、OsP5CS、OsProT和OsLea3等多个胁迫相关基因的表达水平均显著提高。我们的研究表明，ZFP179在植物对盐胁迫的反应中起着至关重要的作用，并有助于开发耐盐性增强的转基因作物。
The Cys2/His2-type zinc finger proteins have been implicated in different cellular processes involved in plant development and stress responses. Through microarray analysis, a salt-responsive zinc finger protein gene ZFP179 was identified and subsequently cloned from rice seedlings. ZFP179 encodes a 17.95 kDa protein with two C2H2-type zinc finger motifs having transcriptional activation activity. The real-time RT-PCR analysis showed that ZFP179 was highly expressed in immature spikes, and markedly induced in the seedlings by NaCl, PEG 6000, and ABA treatments. Overexpression of ZFP179 in rice increased salt tolerance and the transgenic seedlings showed hypersensitivity to exogenous ABA. The increased levels of free proline and soluble sugars were observed in transgenic plants compared to wild-type plants under salt stress. The ZFP179 transgenic rice exhibited significantly increased tolerance to oxidative stress, the reactive oxygen species (ROS)-scavenging ability, and expression levels of a number of stress-related genes, including OsDREB2A, OsP5CS OsProT, and OsLea3 under salt stress. Our studies suggest that ZFP179 plays a crucial role in the plant response to salt stress, and is useful in developing transgenic crops with enhanced tolerance to salt stress.
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影响因子：
7.4
作者：
Sakamoto, H;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
4.9
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
5.1
作者：
Igarashi, Y;Yoshiba, Y;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
4.3
作者：
Lu, Guojun;Gao, Chenxi;Han, Bin
通讯作者：
Han, Bin
影响因子：
3.7
作者：
Jain D;Roy N;Chattopadhyay D
通讯作者：
Chattopadhyay D