Degradation of different pectins by fungi: correlations and contrasts between the pectinolytic enzyme sets identified in genomes and the growth on pectins of different origin.
Degradation of different pectins by fungi: correlations and contrasts between the pectinolytic enzyme sets identified in genomes and the growth on pectins of different origin.
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DOI：
10.1186/1471-2164-13-321
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发表时间：
2012-07-19
期刊：
影响因子：
4.4
通讯作者：
de Vries RP
中科院分区：
文献类型：
--
作者：
Benoit I;Coutinho PM;Schols HA;Gerlach JP;Henrissat B;de Vries RP
果胶是种类繁多且非常复杂的生物分子，其结构取决于植物种类和组织。以往的研究表明，果胶聚合物的衍生物和果胶低聚糖对人体健康有积极的作用。为了获得特定的果胶低聚糖，需要高度确定的酶混合物。丝状真菌是专门降解植物细胞壁的真菌，有些能产生广泛的果胶酶。因此，它们可能会阐明部分水解所需的酶混合物。12种真菌在4种果胶和4种果胶结构元件上的生长情况表明，真菌基因组中果胶分解基因的存在与否与其降解果胶的能力明显相关。然而，当我们放大到果胶结构元素时，这种相关性就不那么明显了。这项研究强调了真菌降解果胶等复杂碳源的复杂机制的复杂性。挖掘基因组和比较基因组学是生产特定果胶分解组分的有希望的第一步。
Pectins are diverse and very complex biomolecules and their structure depends on the plant species and tissue. It was previously shown that derivatives of pectic polymers and oligosaccharides from pectins have positive effects on human health. To obtain specific pectic oligosaccharides, highly defined enzymatic mixes are required. Filamentous fungi are specialized in plant cell wall degradation and some produce a broad range of pectinases. They may therefore shed light on the enzyme mixes needed for partial hydrolysis. The growth profiles of 12 fungi on four pectins and four structural elements of pectins show that the presence/absence of pectinolytic genes in the fungal genome clearly correlates with their ability to degrade pectins. However, this correlation is less clear when we zoom in to the pectic structural elements. This study highlights the complexity of the mechanisms involved in fungal degradation of complex carbon sources such as pectins. Mining genomes and comparative genomics are promising first steps towards the production of specific pectinolytic fractions.
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影响因子：
3.6
作者：
BUCHI, G;LUK, KC;TOWNSEND, JM
通讯作者：
TOWNSEND, JM
影响因子：
12.3
作者：
Espagne E;Lespinet O;Malagnac F;Da Silva C;Jaillon O;Porcel BM;Couloux A;Aury JM;Ségurens B;Poulain J;Anthouard V;Grossetete S;Khalili H;Coppin E;Déquard-Chablat M;Picard M;Contamine V;Arnaise S;Bourdais A;Berteaux-Lecellier V;Gautheret D;de Vries RP;Battaglia E;Coutinho PM;Danchin EG;Henrissat B;Khoury RE;Sainsard-Chanet A;Boivin A;Pinan-Lucarré B;Sellem CH;Debuchy R;Wincker P;Weissenbach J;Silar P
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Silar P
影响因子：
11.2
作者：
Coenen, G. J.;Bakx, E. J.;Voragen, A. G. J.
通讯作者：
Voragen, A. G. J.
影响因子：
5.6
作者：
Eisenhaber, B;Schneider, G;Eisenhaber, F
通讯作者：
Eisenhaber, F
影响因子：
7
作者：
Andersen, Mikael R.;Salazar, Margarita P.;Baker, Scott E.
通讯作者：
Baker, Scott E.