Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells
Dual-core coaxial bioprinting of double-channel constructs with a potential for perfusion and interaction of cells
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双通道结构的双核同轴生物打印具有细胞灌注和相互作用的潜力
DOI：
10.1088/1758-5090/ac6e88
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发表时间：
2022-05
期刊：
影响因子：
9
通讯作者：
Weijie Peng
中科院分区：
文献类型：
--
作者：
Yanrong Yu;Renjian Xie;Yueteng He;Furong Zhao;Quan Zhang;Wei Wang;Yi Zhang;Jiawei Hu;Dan Luo;Weijie Peng
摘要水凝胶管的同轴生物打印在制造高度复杂的大规模血管化结构中具有巨大的潜力，然而，同时具有弱可打印性和可灌注网络的生物墨水的构造尚未报道。在这里，我们报告了一种同轴打印方法，其中使用定制的双核同轴喷嘴对双通道细丝进行三维（3D）挤出生物打印。其中一个通道中的纤维可以发挥核/壳作用，另一个通道可以发挥灌注作用。藻酸盐壁的厚度（约50 μ m）有利于通过灌流补充营养物质。采用不同的弱力学载细胞水凝胶对该方法的适应性和灌流性进行了测试，结果表明动态灌流比静态培养保持了更高的活力和功能。通过与生物打印机相结合，制备了8层可灌注双通道构建体，并且随着下游培养基中营养物质和氧气的减少，细胞活力逐渐下降。以小鼠胰岛素瘤6（Min 6）和肝细胞癌（HepG 2）为模型细胞，通过序贯灌流，将双通道细丝作为模拟细胞间动态功能的平台进行了测试。这些结果表明，由Min 6上游分泌的胰岛素模拟并增加下游HepG 2细胞对葡萄糖的摄取。总之，我们的研究提供了证据的可能性，所有在一个制造的三维双通道灌注结构具有高度的简单性，可扩展性和通用性。我们的策略在构建大规模组织构建物用于组织工程，甚至可能用于药物筛选和再生医学方面具有重要的潜力。
Abstract Coaxial bioprinting of hydrogel tubes has tremendous potential in the fabrication of highly complex large-scale vascularized structures, however, constructs with bioinks of simultaneous weak printability and perfusable networks have not been reported. Here, we report a coaxial printing method in which double-channel filaments are three-dimensional (3D) extrusion-bioprinted using a customized dual-core coaxial nozzle. The filament in one channel can perform core/shell role and the other channel can play a role in perfusion. These parallel channels within filaments are separated by an interval wall of alginate, whose thickness (∼50 μ m) is beneficial to supplement nutrients via perfusion. Different cell-laden hydrogels of weak mechanics were used to test the adaptability and perfusability of our method, and the results showed that dynamic perfusion maintained higher viability and functions than static culture. By combining with a bioprinter, 8-layer perfusable double-channel constructs were fabricated, and the cell viabilities gradually decreased with the reduction in nutrients and oxygen in the downstream medium. Furthermore, the double-channel filaments were tested as a platform to mimic dynamic functions between cells through sequential perfusion by using Mouse insulinoma 6 (Min6) and Hepatocellular carcinoma (HepG2) as the model cells. These results demonstrated the insulin secreted by Min6 upstream simulated and increased the uptake of glucose by the downstream HepG2 cells. In conclusion, our study provided evidence for the probability of all-in-one fabrication of 3D double-channel perfusable constructs with high simplicity, expansibility, and versability. Our strategy has significant potential for building large-scale tissue constructs for applications in tissue engineering, possibly even in drug screening and regenerative medicine.
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影响因子：
9
作者：
Zhang Y;Yu Y;Chen H;Ozbolat IT
通讯作者：
Ozbolat IT
影响因子：
13.3
作者：
Shao, Lei;Gao, Qing;He, Yong
通讯作者：
He, Yong
影响因子：
3
作者：
Lovett, Michael;Rockwood, Danielle;Kaplan, David L.
通讯作者：
Kaplan, David L.
影响因子：
3.1
作者：
O'Neil, RG;Wu, L;Mullani, N
通讯作者：
Mullani, N
影响因子：
29.4
作者：
Szklanny, Ariel A.;Machour, Majd;Levenberg, Shulamit
通讯作者：
Levenberg, Shulamit