Characterization of printable cellular micro-fluidic channels for tissue engineering.
Characterization of printable cellular micro-fluidic channels for tissue engineering.
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DOI：
10.1088/1758-5082/5/2/025004
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发表时间：
2013-06
期刊：
影响因子：
9
通讯作者：
Ozbolat IT
中科院分区：
文献类型：
--
作者：
Zhang Y;Yu Y;Chen H;Ozbolat IT
组织工程一直是一个有前途的研究领域，为弥合器官短缺和移植需求之间的差距提供了希望。然而，构建三维（3D）血管器官仍然是需要克服的主要技术障碍。主要挑战之一是纳入血管网络以支持细胞在营养和氧气灌注方面的活力。本文介绍了一种制造类血管微流体通道的新方法，该方法有可能在未来用于厚组织或器官的制造。在这项研究中，我们研究了可打印微流体通道的可制造性，其中微流体通道支持机械完整性并实现 3D 流体传输。开发了一种压力辅助固体自由成型制造平台，带有同轴针分配器单元，用于打印中空水凝胶丝。研究了点胶流变学，并分析了材料特性对中空丝结构形成的影响。样品结构通过开发的计算机控制系统打印。此外，本文还介绍了细胞活力和基因表达研究。细胞活力表明，软骨祖细胞 (CPC) 在生物打印后和长期体外培养过程中保持了活力。与单层培养的 CPC 相比，实时 PCR 分析发现包裹海藻酸盐中空丝的 CPC 中软骨特异性基因的表达相对较高，这表明可打印的半透性微流体通道为细胞生长和功能提供了理想的环境。
Tissue engineering has been a promising field of research, offering hope of bridging the gap between organ shortage and transplantation needs. However, building three-dimensional (3D) vascularized organs remains the main technological barrier to be overcome. One of the major challenges is the inclusion of a vascular network to support cell viability in terms of nutrients and oxygen perfusion. This paper introduces a new approach to fabrication of vessel-like microfluidic channels that has the potential to be used in thick tissue or organ fabrication in the future. In this research, we investigate the manufacturability of printable micro-fluidic channels, where micro-fluidic channels support mechanical integrity as well as enable fluid transport in 3D. A pressure-assisted solid freeform fabrication platform is developed with a coaxial needle dispenser unit to print hollow hydrogel filaments. The dispensing rheology is studied, and effects of material properties on structural formation of hollow filaments are analyzed. Sample structures are printed through the developed computer-controlled system. In addition, cell viability and gene expression studies are presented in this paper. Cell viability shows that cartilage progenitor cells (CPCs) maintained their viability right after bioprinting and during prolonged in vitro culture. Real-time PCR analysis yielded relatively higher expression of cartilage-specific genes in alginate hollow filament encapsulating CPCs, compared with monolayer cultured CPCs, which revealed that printable semi-permeable micro-fluidic channels provided an ideal environment for cell growth and function.
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