Adhesion and Alignment of Nonparenchymal Cells onto a Patterned Surface with a Two‐Step Plasma Polymerization Process
Adhesion and Alignment of Nonparenchymal Cells onto a Patterned Surface with a Two‐Step Plasma Polymerization Process
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采用两步等离子体聚合工艺将非实质细胞粘附并排列在图案表面上
DOI：
10.1002/ppap.201400173
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发表时间：
2015
影响因子：
3.5
通讯作者：
S. Kudo
中科院分区：
文献类型：
--
作者：
H. Muguruma;T. Hoshino;Ryosuke Fujita;T. Sumii;S. Kudo
对非实质细胞（内皮细胞；EC 和肝星状细胞；HSC）微图案的精确控制对于再生医学、人造器官和基于细胞的生物设备非常有用。研究了非实质细胞在通过两步等离子体聚合工艺制造的微图案表面上的粘附和排列。第一个功能化步骤涉及沉积富氮等离子体聚合膜，以使整个表面成为细胞粘附区域。随后，通过网格金属掩模（数百微米大小的开口）形成疏水性等离子体聚合膜，以使这些区域具有细胞排斥性。成像椭偏仪显示，获得了100μm宽的凹槽和脊图案以及数十纳米级高度的台阶。在图案化表面上进行 EC 和 HSC 培养实验。 EC 沿着图案化表面的细胞粘附凹槽快速粘附并排列，但它不粘附到细胞排斥脊上。仅当细胞排斥脊的高度为20nm时HSC图案化才成功，而当脊高度为10nm时图案化失败。这表明仅通过化学效应即可实现 EC 图案化，而 HSC 图案化则需要化学效应和图案化表面的拓扑约束。
The precise control for the micropatterning of nonparenchymal cells (endothelial cells; ECs and hepatic stellate cells; HSCs) is useful for regenerative medicine, artificial organs, and cell-based biodevice. The adhesion and alignment of nonparenchymal cells on a micro-patterned surface fabricated by two-step plasma polymerization process were investigated. The first functionalization step involves the deposition of a nitrogen-rich plasma-polymerized film to render the entire surface a cell-adherent region. Following this, a hydrophobic plasma-polymerized film is formed through a grid metal mask (hundred-micrometer-sized openings) to renders these areas cell repellent. Imaging ellipsometry showed that groove and ridge patterns 100 μm wide and steps with heights on the scale of tens of nanometers were obtained. EC and HSC culture experiments were conducted on the patterned surfaces. EC rapidly adhered and aligned along the cell-adherent groove of the patterned surface, while it did not adhere to the cell-repellent ridge. HSC patterning succeeded only when the height of the cell-repellent ridge was 20 nm, whereas the patterning failed when the ridge height was 10 nm. This indicates that EC patterning is possible with only a chemical effect, whereas HSC patterning required both a chemical effect and the topological constraints of the patterned surface.
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电纺聚合物支架的微尺度等离子体引发图案化。
DOI：
10.1016/j.colsurfb.2011.01.014
发表时间：
2011
期刊：
Colloids and surfaces. B, Biointerfaces
影响因子：
--
作者：
Delgado-Rivera,Roberto;Griffin,Jeremy;Ricupero,ChristopherL;Grumet,Martin;Meiners,Sally;Uhrich,KathrynE
通讯作者：
Uhrich,KathrynE
DOI：
10.1016/s0006-291x(03)01165-3
发表时间：
2003-07-25
影响因子：
3.1
作者：
Chen, CS;Alonso, JL;Ingber, DE
通讯作者：
Ingber, DE
DOI：
10.1002/(sici)1097-4636(199602)30:2
发表时间：
1996-02
期刊：
Journal of biomedical materials research
影响因子：
--
作者：
K. Kottke-Marchant;A. Veenstra;R. Marchant
通讯作者：
K. Kottke-Marchant;A. Veenstra;R. Marchant
影响因子：
7.4
作者：
Khademhosseini, A;Suh, KY;Langer, R
通讯作者：
Langer, R