Degradation or excretion of quantum dots in mouse embryonic stem cells.
Degradation or excretion of quantum dots in mouse embryonic stem cells.
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DOI：
10.1186/1472-6750-10-36
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发表时间：
2010-05-06
影响因子：
3.5
通讯作者：
Cao Y
中科院分区：
文献类型：
--
作者：
Pi QM;Zhang WJ;Zhou GD;Liu W;Cao Y
量子点（QD）被认为是一种在体外和体内非侵入性标记细胞的新型有效探针，但人们对标记后如何从细胞中消除 QD 知之甚少。本研究的目的是研究量子点在不同类型细胞中的代谢。用 QD 655 标记小鼠胚胎干细胞 (ESC) 和小鼠胚胎成纤维细胞 (MEF)。通过荧光显微镜和流式细胞术监测 QD 标记 72 小时。两种类型的细胞都被有效标记，但在 48 小时内观察到 ESC 中 QD 标记的快速丢失，而抑制细胞增殖并不能阻止这种现象。透射电子显微镜分析显示，标记后 24 小时，ESC 囊泡中的 QD 数量急剧减少，表明 QD 可能被降解。此外，从培养中标记的ESC收集的上清液再次用于标记细胞，表明一些QD从细胞中分泌出来。这是第一个研究证明量子点在不同类型细胞中的代谢是不同的。标记后，QD 很快被降解或从 ESC 中排出。
Quantum dots (QDs) have been considered as a new and efficient probe for labeling cells non-invasively in vitro and in vivo, but fairly little is known about how QDs are eliminated from cells after labeling. The purpose of this study is to investigate the metabolism of QDs in different type of cells. Mouse embryonic stem cells (ESCs) and mouse embryonic fibroblasts (MEFs) were labeled with QD 655. QD-labeling was monitored by fluorescence microscopy and flow cytometry for 72 hours. Both types of cells were labeled efficiently, but a quick loss of QD-labeling in ESCs was observed within 48 hours, which was not prevented by inhibiting cell proliferation. Transmission electron microscope analysis showed a dramatic decrease of QD number in vesicles of ESCs at 24 hours post-labeling, suggesting that QDs might be degraded. In addition, supernatants collected from labeled ESCs in culture were used to label cells again, indicating that some QDs were excreted from cells. This is the first study to demonstrate that the metabolism of QDs in different type of cells is different. QDs were quickly degraded or excreted from ESCs after labeling.
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