A novel behavioral assay for the assessment of acute tinnitus in rats optimized for simultaneous recording of oscillatory neural activity.
A novel behavioral assay for the assessment of acute tinnitus in rats optimized for simultaneous recording of oscillatory neural activity.
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DOI：
10.1016/j.jneumeth.2013.07.021
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发表时间：
2013-10-15
影响因子：
3
通讯作者：
Allman, Brian L.
中科院分区：
文献类型：
--
作者：
Stolzberg, Daniel;Hayes, Sarah H.;Kashanian, Nina;Radziwon, Kelly;Salvi, Richard J.;Allman, Brian L.
人类磁/电生理学研究表明，耳鸣的幻音来自听觉皮层中的自发振荡神经活动;然而，在动物模型中，适合于结合电生理学记录来测试这一假设的行为技术还有待评估。虽然耳鸣的电生理学研究已经在被动的清醒动物中报道，但这些研究未能控制可能在耳鸣感知中发挥作用的注意力机制。本研究开发了一种新的食欲操作性条件反射-两种选择识别任务，用于检测大鼠急性耳鸣。该程序优化了同时记录振荡神经活动的条件，同时控制动物的注意力状态。在全身注射水杨酸钠（200 mg/kg IP）（一种已知的耳鸣诱导剂）后，在7只大鼠中的6只中检测到耳鸣。对长期植入耳鸣大鼠听觉皮层的电极记录的持续局部场电位进行分析，发现持续神经活动频谱发生变化。现有的耳鸣检测方法没有明确设计用于同时记录神经活动。这里报道的行为方法是第一个提供在慢性植入大鼠中获得这些记录所需的条件。这里提出的行为分析将有助于研究耳鸣的神经机制，使研究人员能够比较确认耳鸣动物的电生理数据。
Human magneto/electrophysiology studies suggest that the phantom sound of tinnitus arises from spontaneous oscillatory neural activity in auditory cortex; however, in animal models, behavioral techniques suitable for testing this hypothesis in combination with electrophysiology recordings have yet to be evaluated. While electrophysiological studies of tinnitus have been reported in passive, awake animals, these studies fail to control for attentional mechanisms likely to play a role in the perception of tinnitus. A novel appetitive operant conditioning, two-alternative identification task was developed for detecting acute tinnitus in rats. The procedure optimizes conditions for simultaneously recording oscillatory neural activity while controlling for the attentional state of the animal. Tinnitus was detected in six of seven rats following systemic injection with sodium salicylate (200 mg/kg IP), a known inducer of tinnitus. Analysis of ongoing local field potentials recorded from chronically implanted electrodes in auditory cortex of a rat reporting tinnitus revealed changes in the spectrum of ongoing neural activity. Existing tinnitus-detection methods were not explicitly designed for the simultaneous recording of neural activity. The behavioral method reported here is the first to provide the conditions necessary for obtaining these recordings in chronically implanted rats. The behavioral assay presented here will facilitate research into the neural mechanisms of tinnitus by allowing researchers to compare the electrophysiological data in animals with confirmed tinnitus.
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细胞外田地和电流的起源-EEG，ECOG，LFP和尖峰。
DOI：
10.1038/nrn3241
发表时间：
2012-05-18
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
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通讯作者：
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影响因子：
3
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SASAKI, CT
影响因子：
120.7
作者：
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通讯作者：
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影响因子：
4
作者：
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通讯作者：
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