Self-assembling nanocomplexes by combining ferumoxytol, heparin and protamine for cell tracking by magnetic resonance imaging.
Self-assembling nanocomplexes by combining ferumoxytol, heparin and protamine for cell tracking by magnetic resonance imaging.
复制标题
作者：
我们报道了一种新颖而简单的磁性细胞标记方法，它结合了FDA批准的三种药物，阿魏酸甘油(F)、肝素(H)和鱼精蛋白(P)在无血清介质中形成自组装纳米复合体，有效地标记细胞用于体内MRI。我们观察到HPF纳米络合物在无血清细胞培养液中是稳定的。与F相比，HPF纳米复合体的T2弛豫度增加了三倍。电子显微镜显示HPF内在化，标记细胞的普鲁士蓝染色证实了这一点。与对照组相比，HPF标记的造血干细胞、骨髓基质细胞、神经干细胞和T细胞对细胞生理或功能没有长期影响或毒性。活体核磁共振检测到1000个HPF标记细胞植入大鼠脑内。HPF标记方法有助于临床试验中输注或植入细胞的MRI监测。
We report on a novel and straightforward magnetic cell labeling approach that combines three FDA-approved drugs, ferumoxytol (F), heparin (H) and protamine (P) in serum free media to form self-assembling nanocomplexes that effectively label cells for in vivo MRI. We observed that the HPF nanocomplexes were stable in serum free cell culture media. HPF nanocomplexes exhibited a three-fold increase in T2 relaxivity compared to F. Electron Microscopy revealed internalized HPF within endosomes, confirmed by Prussian blue staining of labeled cells. There was no long-term effect or toxicity on cellular physiology or function of HPF-labeled hematopoietic stem cells, bone marrow stromal cells, neural stem cells, and T-cells when compared to controls. In vivo MRI detected 1000 HPF-labeled cells implanted in rat brains. HPF labeling method should facilitate the monitoring by MRI of infused or implanted cells in clinical trials.
登录
查看更多内容
影响因子：
10.8
作者：
Liang, JF;Li, YT;Yang, VC
通讯作者：
Yang, VC
影响因子：
--
作者：
Boutry, Sebastien;Brunin, Stephanie;Muller, Robert N.
通讯作者：
Muller, Robert N.
影响因子：
2.9
作者：
Pawelczyk, Edyta;Arbab, Ali S.;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
影响因子：
3.3
作者：
Liu, Wei;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
影响因子：
2.8
作者：
Arbab, Ali Syed;Yocum, Gene Thomus;Frank, Joseph Alan
通讯作者：
Frank, Joseph Alan