Real-time environmental surveillance of SARS-CoV-2 aerosols.
Real-time environmental surveillance of SARS-CoV-2 aerosols.
复制标题
SARS-CoV-2 气溶胶的实时环境监测。
DOI：
10.1038/s41467-023-39419-z
复制
发表时间：
2023-07-10
影响因子：
16.6
通讯作者：
Chakrabarty, Rajan K. K.
中科院分区：
文献类型：
--
作者：
Puthussery, Joseph V. V.;Ghumra, Dishit P. P.;McBrearty, Kevin R. R.;Doherty, Brookelyn M. M.;Sumlin, Benjamin J. J.;Sarabandi, Amirhossein;Mandal, Anushka Garg;Shetty, Nishit J. J.;Gardiner, Woodrow D. D.;Magrecki, Jordan P. P.;Brody, David L. L.;Esparza, Thomas J. J.;Bricker, Traci L. L.;Boon, Adrianus C. M.;Yuede, Carla M. M.;Cirrito, John R. R.;Chakrabarty, Rajan K. K.
自 COVID-19 大流行开始以来，对空气传播的 SARS-CoV-2 病毒进行实时监测是科学界一直未能解决的技术空白。用于 SARS-CoV-2 检测的离线空气采样技术的周转时间较长，并且需要熟练的劳动力。在这里，我们提出了一种概念验证的病原体空气质量 (pAQ) 监测器，用于实时（5 分钟时间分辨率）直接检测 SARS-CoV-2 气溶胶。该系统协同集成了高流量（~1000 lpm）湿旋风空气采样器和基于纳米体的超灵敏微免疫电极生物传感器。湿旋风分离器显示出与市售采样器相当或更好的病毒采样性能。实验室实验表明，设备灵敏度为 77-83%，检测限为 7-35 个病毒 RNA 拷贝/立方米空气。我们的 pAQ 监测仪适用于室内环境中 SARS-CoV-2 变体的按需监测，并且可适用于其他感兴趣的呼吸道病原体的多重检测。广泛采用此类技术可以帮助公共卫生官员实施快速疾病控制措施。快速检测室内环境中循环的呼吸道病原体可以促进改善感染预防反应。在这项概念验证研究中，作者开发了一种病原体空气质量监测器，用于实时直接检测 SARS-CoV-2 气溶胶，并展示了其在 SARS-CoV-2 感染者房间中的应用。
Real-time surveillance of airborne SARS-CoV-2 virus is a technological gap that has eluded the scientific community since the beginning of the COVID-19 pandemic. Offline air sampling techniques for SARS-CoV-2 detection suffer from longer turnaround times and require skilled labor. Here, we present a proof-of-concept pathogen Air Quality (pAQ) monitor for real-time (5 min time resolution) direct detection of SARS-CoV-2 aerosols. The system synergistically integrates a high flow (~1000 lpm) wet cyclone air sampler and a nanobody-based ultrasensitive micro-immunoelectrode biosensor. The wet cyclone showed comparable or better virus sampling performance than commercially available samplers. Laboratory experiments demonstrate a device sensitivity of 77–83% and a limit of detection of 7-35 viral RNA copies/m3 of air. Our pAQ monitor is suited for point-of-need surveillance of SARS-CoV-2 variants in indoor environments and can be adapted for multiplexed detection of other respiratory pathogens of interest. Widespread adoption of such technology could assist public health officials with implementing rapid disease control measures. Rapid detection of respiratory pathogens circulating in indoor environments could facilitate improved infection prevention responses. In this proof-of-concept study, the authors develop a pathogen air quality monitor for real-time direct detection of SARS-CoV-2 aerosols and demonstrate its application in rooms of people with SARS-CoV-2 infections.
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DOI：
10.1080/02786826.2014.881460
发表时间：
2014-01-01
期刊：
Aerosol science and technology : the journal of the American Association for Aerosol Research
影响因子：
--
作者：
Hering SV;Spielman SR;Lewis GS
通讯作者：
Lewis GS
影响因子：
4.7
作者：
Prabhulkar S;Piatyszek R;Cirrito JR;Wu ZZ;Li CZ
通讯作者：
Li CZ
影响因子：
8.4
作者：
Lednickya, John A.;Lauzardo, Michael;Wu, Chang-Yu
通讯作者：
Wu, Chang-Yu
影响因子：
12.6
作者：
Agarwal DK;Nandwana V;Henrich SE;Josyula VPVN;Thaxton CS;Qi C;Simons LM;Hultquist JF;Ozer EA;Shekhawat GS;Dravid VP
通讯作者：
Dravid VP
影响因子：
8.9
作者：
Giovannini G;Haick H;Garoli D
通讯作者：
Garoli D