A genome-wide library of CB4856/N2 introgression lines of Caenorhabditis elegans.
A genome-wide library of CB4856/N2 introgression lines of Caenorhabditis elegans.
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DOI：
10.1093/nar/gkp528
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发表时间：
2009-09
影响因子：
14.9
通讯作者：
Kammenga J
中科院分区：
文献类型：
--
作者：
Doroszuk A;Snoek LB;Fradin E;Riksen J;Kammenga J
来源于秀丽隐杆线虫野生型N2和CB4856的重组近交系（RILs）越来越多地用于绘制复杂性状的基因。为了加快作图和基因发现的速度，渐渗系（IL）为更有效的QTL鉴定提供了强有力的工具。我们构建了90个IL的文库，每个IL携带渗入到N2的遗传背景中的单个纯合CB4856基因组片段。通过123个单核苷酸多态性（SNP）标记对IL进行基因分型。大多数品系中CB4856区段的比例不超过3%，并且基因渗入一起覆盖了CB4856基因组的96%。IL文库的价值通过鉴定两个衰老相关性状（即寿命和咽泵率）中潜在的自然变异的新基因座来证明。寿命的Bin作图得到6个QTL，它们都对CB4856等位基因具有寿命缩短效应。我们发现了5个泵送速率下降的QTL，其中4个与平均寿命的QTL共位。这表明与寿命和泵送速率相关的多效或紧密连锁的QTL。总的来说，IL文库提供了一个通用的资源，更容易和有效的精细定位和功能分析的基因座和基因的复杂性状在C。优雅的
Recombinant inbred lines (RILs) derived from Caenorhabditis elegans wild-type N2 and CB4856 are increasingly being used for mapping genes underlying complex traits. To speed up mapping and gene discovery, introgression lines (ILs) offer a powerful tool for more efficient QTL identification. We constructed a library of 90 ILs, each carrying a single homozygous CB4856 genomic segment introgressed into the genetic background of N2. The ILs were genotyped by 123 single-nucleotide polymorphism (SNP) markers. The proportion of the CB4856 segments in most lines does not exceed 3%, and together the introgressions cover 96% of the CB4856 genome. The value of the IL library was demonstrated by identifying novel loci underlying natural variation in two ageing-related traits, i.e. lifespan and pharyngeal pumping rate. Bin mapping of lifespan resulted in six QTLs, which all have a lifespan-shortening effect on the CB4856 allele. We found five QTLs for the decrease in pumping rate, of which four colocated with QTLs found for average lifespan. This suggests pleiotropic or closely linked QTL associated with lifespan and pumping rate. Overall, the presented IL library provides a versatile resource toward easier and efficient fine mapping and functional analyses of loci and genes underlying complex traits in C. elegans.
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DOI：
10.1126/science.1166527
发表时间：
2009-01-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Reddy KC;Andersen EC;Kruglyak L;Kim DH
通讯作者：
Kim DH
DOI：
10.1073/pnas.0400848101
发表时间：
2004-05-25
影响因子：
11.1
作者：
Huang, C;Xiong, CJ;Kornfeld, K
通讯作者：
Kornfeld, K
DOI：
10.1073/pnas.76.3.1333
发表时间：
1979-01-01
影响因子：
11.1
作者：
EMMONS, SW;KLASS, MR;HIRSH, D
通讯作者：
HIRSH, D
DOI：
10.1073/pnas.0601893103
发表时间：
2006-05-16
影响因子：
11.1
作者：
Kristensen, Vessela N.;Edvardsen, Hege;Borresen-Dale, Anne-Lise
通讯作者：
Borresen-Dale, Anne-Lise
在秀丽隐杆线虫中，基因基因组学对基因表达可塑性的映射决定因素。
影响因子：
4.5
作者：
Li, Yang;Alvarez, Olga Alda;Gutteling, Evert W.;Tijsterman, Marcel;Fu, Jingyuan;Riksen, Joost A. G.;Hazendonk, Esther;Prins, Pjotr;Plasterk, Ronald H. A.;Jansen, Ritsert C.;Breitling, Rainer;Kammenga, Jan E.
通讯作者：
Kammenga, Jan E.