Histopathology and Ultrastructural Findings of Fatal COVID-19 Infections on Testis.
Histopathology and Ultrastructural Findings of Fatal COVID-19 Infections on Testis.
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DOI：
10.5534/wjmh.200170
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Ramasamy R
中科院分区：
文献类型：
--
作者：
Achua JK;Chu KY;Ibrahim E;Khodamoradi K;Delma KS;Iakymenko OA;Kryvenko ON;Arora H;Ramasamy R
评估严重急性呼吸综合征冠状病毒2型（COVID-19）并发症死亡或康复患者睾丸内的病理变化并进行分析。从COVID-19阳性（n=6）和阴性男性（n=3）的尸检中收集睾丸组织。福尔马林固定的石蜡包埋组织用苏木精和伊红（H&E）染色，并进行血管紧张素转换酶2（ACE-2）表达的免疫荧光。荧光标记的组织载玻片在定量病理学显微镜上成像，具有各种缩放水平，允许定性和定量解释。来自四个COVID-19阳性尸检病例和一个活的血清转化患者的组织用透射电子显微镜（TEM）成像。H&E组织形态学显示，6例COVID-19活组织检查中有3例精子发生正常，其余3例精子发生受损。透射电镜显示1例COVID-19阳性尸检病例的睾丸组织中存在COVID-19病毒，同一尸检病例的活体活检、H&E染色显示间质巨噬细胞和白细胞浸润。来自6名COVID-19阳性男性的免疫荧光染色载玻片显示，ACE-2定量水平升高与精子发生受损之间存在直接关联。新型COVID-19对ACE-2受体具有亲和力。由于ACE-2受体在睾丸中表达较高，我们假设COVID-19在感染患者的睾丸组织中普遍存在。这项研究表明，男性生殖道，特别是睾丸，可能是COVID-19感染的目标。我们发现ACE-2受体水平与精子发生之间存在负相关，这表明COVID-19可能导致不育的可能机制。
To evaluate the presence and analyze the pathological changes within the testes of patients who died or recovered from severe acute respiratory syndrome coronavirus 2 (COVID-19) complications. Testis tissue was collected from autopsies of COVID-19 positive (n=6) and negative men (n=3). Formalin-fixed paraffin-embedded tissues were stained with hematoxylin and eosin (H&E) and subjected to immunofluorescence for angiotensin-converting enzyme 2 (ACE-2) expression. Fluorescent-labeled tissue slides were imaged on a quantitative pathology scope with various zoom levels allowing for qualitative and quantitative interpretation. Tissue from four COVID-19 positive autopsy cases and a live seroconverted patient was imaged with transmission electron microscopy (TEM). H&E histomorphology showed three of the six COVID-19 biopsies had normal spermatogenesis while the remaining three had impaired spermatogenesis. TEM showed the COVID-19 virus in testis tissue of one COVID-19 positive autopsy case and the live biopsy, H&E stain on the same autopsy case demonstrated interstitial macrophage and leukocyte infiltration. Immunofluorescent stained slides from six COVID-19 positive men demonstrated a direct association between increased quantitative ACE-2 levels and impairment of spermatogenesis. The novel COVID-19 has an affinity for ACE-2 receptors. Since ACE-2 receptor expression is high in the testes, we hypothesized that COVID-19 is prevalent in testes tissue of infected patients. This study suggests the male reproductive tract, specifically the testes, may be targets of COVID-19 infection. We found an inverse association between ACE-2 receptor levels and spermatogenesis, suggesting a possible mechanism of how COVID-19 can cause infertility.
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DOI：
10.1016/j.ancard.2020.09.030
发表时间：
2020-11
期刊：
Annales de cardiologie et d'angeiologie
影响因子：
--
作者：
Georges JL;Cochet H;Roger G;Ben Jemaa H;Soltani J;Azowa JB;Mamou R;Gilles F;Saba J;Prevot A;Pasqualini M;Monguillon V;De Tournemire M;Bertrand A;Koukabi-Fradelizi M;Beressi JP;Livarek B
通讯作者：
Livarek B
影响因子：
13.6
作者：
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通讯作者：
Jentzen, Jeffrey M.
影响因子：
5.4
作者：
Paoli D;Pallotti F;Nigro G;Aureli A;Perlorca A;Mazzuti L;Di Carlo D;Turriziani O;Lenzi A;Lombardo F
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Lombardo F
影响因子：
6.1
作者：
McLachlan, R. I.;Rajpert-De Meyts, E.;Skakkebaek, N. E.
通讯作者：
Skakkebaek, N. E.
影响因子：
1.9
作者：
Wang, Wen;Zhu, Yueke;Li, Wei
通讯作者：
Li, Wei