Influence of Intestinal Indigenous Microbiota on Intrafamilial Infection by Helicobacter pylori in Japan.
Influence of Intestinal Indigenous Microbiota on Intrafamilial Infection by Helicobacter pylori in Japan.
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DOI：
10.3389/fimmu.2018.00287
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发表时间：
2018
影响因子：
7.3
通讯作者：
Kamiya S
中科院分区：
文献类型：
--
作者：
Osaki T;Zaman C;Yonezawa H;Lin Y;Okuda M;Nozaki E;Hojo F;Kurata S;Hanawa T;Kikuchi S;Kamiya S
幽门螺杆菌是慢性胃炎、胃溃疡和胃癌的致病菌。已知人类是H的天然宿主。幽门螺杆菌，并倾向于在5岁之前获得病原体。如果不进行根除治疗，感染可能会持续终生。H.幽门螺杆菌是家庭成员之间的疾病，因此，家庭成员感染幽门螺杆菌是儿童感染的一个重要危险因素。然而，其他环境因素尚未得到充分分析。本研究旨在阐明肠道菌群是否以及在多大程度上影响H。幽门螺旋杆菌家庭内感染。从H. pylori感染的婴儿和H. 2010年至2013年，在兵库县佐山市进行的队列研究中收集了幽门螺杆菌感染和未感染的家庭成员。共分析了来自5个家庭的18个粪便DNA。使用16S rRNA通用引物扩增样品，并使用Ion PGM系统对扩增子进行测序。主坐标分析表明，不同品种间肠道菌群无差异。幽门螺杆菌阳性和H.幽门阴性组。在家族内比较试验中，H. pylori感染的婴儿先证者和H.在肠道微生物多样性低的家庭中，母亲幽门螺杆菌阴性的情况最接近。而在肠道微生物多样性最高的家系中，最接近的曼哈顿距离出现在H. pylori感染的婴儿先证者和H.幽门感染的母亲这项研究的结果表明，同一家族的成员之间肠道微生物群的组成非常相似，因此，与感染父母高度相似的微生物定植可能是H.儿童幽门螺杆菌感染
Helicobacter pylori is a causative pathogen of chronic gastritis, gastric ulcer disease, and gastric cancer. Humans are known to be a natural host for H. pylori and tend to acquire the pathogen before the age of 5 years. The infection may then persist lifelong if eradication therapy is not applied. One of the modes of transmission of H. pylori is between family members, and therefore, the presence of infected family members is an important risk factor in children. However, other environmental factors have not been fully analyzed. The present study was performed to clarify whether and to what extent intestinal microbiota affect H. pylori intrafamilial infection. The fecal specimens from H. pylori-infected infants and H. pylori-infected and non-infected family members were collected in cohort studies conducted by Sasayama City, Hyogo Prefecture from 2010 to 2013. In total, 18 fecal DNA from 5 families were analyzed. Samples were amplified using 16S rRNA universal primers, and the amplicons were sequenced using the Ion PGM system. Principal-coordinate analysis demonstrated that there was no difference in intestinal microbiota between H. pylori-positive and H. pylori-negative groups. In intrafamilial comparison tests, the Manhattan distance of intestinal microbiota between the H. pylori-infected infant proband and H. pylori-negative mother was nearest in the family with low intestinal microbial diversity. However, in the family with the highest intestinal microbial diversity, the nearest Manhattan distance was shown between the H. pylori-infected infant proband and H. pylori-infected mother. The results in this study showed that the composition of the intestinal microbiota was very similar between members of the same family, and as such, colonization with organisms highly similar to the infected parent(s) may be a risk factor for H. pylori infection in children.
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影响因子：
3.8
作者：
Osaki, Takako;Matsuki, Takahiro;Kamiya, Shigeru
通讯作者：
Kamiya, Shigeru
影响因子：
3.7
作者：
Jünemann S;Prior K;Szczepanowski R;Harks I;Ehmke B;Goesmann A;Stoye J;Harmsen D
通讯作者：
Harmsen D
影响因子：
3.7
作者：
Furuta Y;Konno M;Osaki T;Yonezawa H;Ishige T;Imai M;Shiwa Y;Shibata-Hatta M;Kanesaki Y;Yoshikawa H;Kamiya S;Kobayashi I
通讯作者：
Kobayashi I
影响因子：
3.6
作者：
Konno, Mutsuko;Yokota, Shin-ichi;Fujii, Nobuhiro
通讯作者：
Fujii, Nobuhiro
影响因子：
29.4
作者：
MARSHALL, BJ;BARRETT, LJ;GUERRANT, RL
通讯作者：
GUERRANT, RL