Chaetognath transcriptome reveals ancestral and unique features among bilaterians.
Chaetognath transcriptome reveals ancestral and unique features among bilaterians.
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DOI：
10.1186/gb-2008-9-6-r94
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发表时间：
2008
期刊：
影响因子：
12.3
通讯作者：
Le Parco, Yannick
中科院分区：
文献类型：
--
作者：
Marletaz, Ferdinand;Gilles, Andre;Caubit, Xavier;Perez, Yvan;Dossat, Carole;Samain, Sylvie;Gyapay, Gabor;Wincker, Patrick;Le Parco, Yannick
chætognath转录组揭示了不寻常的基因组特征，在这个原口动物的进化，并建议它可以作为一个模式生物bilaterians。由于其惊人的形态和发育特征，chætognaths（箭蠕虫）多年来一直困扰着动物学家。尽管它们的发育类似于后口动物，但最近的基因组研究将毛颚动物门定位于原口动物，最有可能是早期分支。这一关键的系统发育位置和毛颚类的独特特征促使人们进一步研究它们的基因组特征。通过对表达序列标签和基因组细菌人工染色体克隆的测序，从chætognath Spadella cephaloptera收集转录组和基因组数据。在不同的分类尺度上的转录本比较强调了核心基因集的保守性，并且基因组分析证实了原口类中毛颚类的基础位置。对转录本多样性和个体基因分型的详细调查显示，在chætognath谱系中，过去发生过基因组重复事件，令人惊讶的是，重复基因的留存率很高。此外，显着的遗传异质性内检测到的抽样人口在核和线粒体水平，但不能解释的神秘物种。最后，我们发现的证据，转录本的反式剪接成熟通过剪接前导除了在chætognath门，我们进一步报告说，这种处理与操纵子转录。这些发现揭示了chætognath基因组的共同祖先和独特的衍生特征，这表明该基因组可能是非常原始的进化历史的产物。这些特征使毛颚类成为比较基因组学研究的重要模式动物，为研究动物基因组的进化提供了新的线索。
The chætognath transcriptome reveals unusual genomic features in the evolution of this protostome and suggests that it could be used as a model organism for bilaterians. The chætognaths (arrow worms) have puzzled zoologists for years because of their astonishing morphological and developmental characteristics. Despite their deuterostome-like development, phylogenomic studies recently positioned the chætognath phylum in protostomes, most likely in an early branching. This key phylogenetic position and the peculiar characteristics of chætognaths prompted further investigation of their genomic features. Transcriptomic and genomic data were collected from the chætognath Spadella cephaloptera through the sequencing of expressed sequence tags and genomic bacterial artificial chromosome clones. Transcript comparisons at various taxonomic scales emphasized the conservation of a core gene set and phylogenomic analysis confirmed the basal position of chætognaths among protostomes. A detailed survey of transcript diversity and individual genotyping revealed a past genome duplication event in the chætognath lineage, which was, surprisingly, followed by a high retention rate of duplicated genes. Moreover, striking genetic heterogeneity was detected within the sampled population at the nuclear and mitochondrial levels but cannot be explained by cryptic speciation. Finally, we found evidence for trans-splicing maturation of transcripts through splice-leader addition in the chætognath phylum and we further report that this processing is associated with operonic transcription. These findings reveal both shared ancestral and unique derived characteristics of the chætognath genome, which suggests that this genome is likely the product of a very original evolutionary history. These features promote chætognaths as a pivotal model for comparative genomics, which could provide new clues for the investigation of the evolution of animal genomes.
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影响因子：
2.8
作者：
Harzsch S;Müller CH
通讯作者：
Müller CH
影响因子：
4.5
作者：
Guiliano DB;Blaxter ML
通讯作者：
Blaxter ML
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
48
作者：
Garcia, Ana Maria;Derventzi, Anastasia;Vijg, Jan
通讯作者：
Vijg, Jan
影响因子：
64.5
作者：
Denes, Alexandru S.;Jekely, Gaspar;Arendt, Detlev
通讯作者：
Arendt, Detlev