A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format.
A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format.
复制标题
DOI：
10.1038/srep37638
复制
发表时间：
2016-11-24
影响因子：
4.6
通讯作者：
Piletsky S
中科院分区：
文献类型：
--
作者：
Smolinska-Kempisty K;Guerreiro A;Canfarotta F;Cáceres C;Whitcombe MJ;Piletsky S
在这里，我们表明，分子印迹聚合物纳米粒子，在水介质中制备的固相合成固定化的L-甲状腺素，葡萄糖胺，伏马菌素B2或生物素作为模板，可以证明可比或更好的性能，商业生产的抗体在酶联竞争性测定。基于印迹纳米颗粒的测定显示检测限在pM范围内，并且聚合物涂覆的微孔板在室温下储存至少1个月是稳定的。在对照实验中，用不相关的模板（胰蛋白酶）印迹的纳米颗粒，但用相同的聚合物组合物制备，未检测到对分析物的响应。固相合成印迹纳米颗粒的制备简便、高亲和力以及不需要冷链物流，使其成为用于免疫测定的传统抗体的有吸引力的替代品。
Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays.
登录
查看更多内容
影响因子：
3.9
作者：
Poma A;Guerreiro A;Caygill S;Moczko E;Piletsky S
通讯作者：
Piletsky S
影响因子：
4.3
作者：
Bedwell TS;Whitcombe MJ
通讯作者：
Whitcombe MJ
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
6.7
作者：
Moczko E;Poma A;Guerreiro A;Perez de Vargas Sansalvador I;Caygill S;Canfarotta F;Whitcombe MJ;Piletsky S
通讯作者：
Piletsky S
影响因子：
4.9
作者：
Suriyanarayanan, Subramanian;Petrone, Luigi;Nicholls, Ian A.
通讯作者：
Nicholls, Ian A.