CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells.
CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells.
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DOI：
10.1084/jem.20091964
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发表时间：
2010-01-18
期刊：
影响因子：
--
通讯作者：
Malissen B
中科院分区：
文献类型：
--
作者：
Henri S;Poulin LF;Tamoutounour S;Ardouin L;Guilliams M;de Bovis B;Devilard E;Viret C;Azukizawa H;Kissenpfennig A;Malissen B
最近的研究挑战了朗格汉斯细胞（LC）构成皮肤的唯一抗原呈递细胞的观点，并表明真皮树突状细胞（DDC）网络非常复杂。使用敲入小鼠跟踪和消融表达langerin（CD 207）的DC，我们发现真皮包含五个不同的DC亚群，并确定了它们在引流淋巴结中的迁移对应物。基于这种精细的分类，我们证明了数量上次要的CD 207 + CD 103 + DDC亚群被赋予了由角质形成细胞表达的交叉呈递抗原的独特能力，而与LC的存在无关。我们进一步表明，Y-Ae（一种广泛用于监测涉及I-Ab分子和I-E α链衍生肽的复合物形成的抗体）识别在I-E α不存在的情况下表达I-Ab分子的成熟皮肤DC。了解这种额外的反应性很重要，因为它可能被错误地解释为支持抗原转移可以在LC和DDC之间发生的观点。总的来说，这些数据重新审视了发生在角质形成细胞和五个可区分的皮肤DC亚群之间的抗原转移，并强调了它们之间存在的高度功能专业化。
Recent studies have challenged the view that Langerhans cells (LCs) constitute the exclusive antigen-presenting cells of the skin and suggest that the dermal dendritic cell (DDC) network is exceedingly complex. Using knockin mice to track and ablate DCs expressing langerin (CD207), we discovered that the dermis contains five distinct DC subsets and identified their migratory counterparts in draining lymph nodes. Based on this refined classification, we demonstrated that the quantitatively minor CD207+ CD103+ DDC subset is endowed with the unique capability of cross-presenting antigens expressed by keratinocytes irrespective of the presence of LCs. We further showed that Y-Ae, an antibody that is widely used to monitor the formation of complexes involving I-Ab molecules and a peptide derived from the I-E α chain, recognizes mature skin DCs that express I-Ab molecules in the absence of I-E α. Knowledge of this extra reactivity is important because it could be, and already has been, mistakenly interpreted to support the view that antigen transfer can occur between LCs and DDCs. Collectively, these data revisit the transfer of antigen that occurs between keratinocytes and the five distinguishable skin DC subsets and stress the high degree of functional specialization that exists among them.
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DOI：
10.1084/jem.20071966
发表时间：
2007-12-24
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bursch LS;Wang L;Igyarto B;Kissenpfennig A;Malissen B;Kaplan DH;Hogquist KA
通讯作者：
Hogquist KA
DOI：
10.1084/jem.192.12.1685
发表时间：
2000-12-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
den Haan JM;Lehar SM;Bevan MJ
通讯作者：
Bevan MJ
影响因子：
30.5
作者：
Liu, Kang;Waskow, Claudia;Nussenzweig, Michel
通讯作者：
Nussenzweig, Michel
DOI：
10.1073/pnas.0812247106
发表时间：
2009-02-03
影响因子：
11.1
作者：
Idoyaga, Juliana;Suda, Nao;Steinman, Ralph M.
通讯作者：
Steinman, Ralph M.
影响因子：
30.5
作者：
Bedoui, Sammy;Whitney, Paul G.;Heath, William R.
通讯作者：
Heath, William R.