Cellular scale anisotropic topography guides Schwann cell motility.
Cellular scale anisotropic topography guides Schwann cell motility.
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DOI：
10.1371/journal.pone.0024316
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hoffman-Kim D
中科院分区：
文献类型：
--
作者：
Mitchel JA;Hoffman-Kim D
雪旺细胞（SC）的定向迁移对于周围神经系统的发育和修复至关重要。了解特定于SC的运动性方面，沿着SC对工程生物材料的反应，可以为增强神经再生的策略提供信息。将大鼠SC培养在层粘连蛋白包被的微槽聚（二甲基硅氧烷）平台上，该平台是平坦的或呈现重复的细胞尺度各向异性地形线索，宽度为30或60 µm，并用时移显微镜观察。SC运动的方向平行于长轴的地形上的凹槽地板和高原，伴随的速度和方向持久性的差异相比，SC运动在平坦的基板上。此外，特征尺寸影响SC形态，对齐，和方向持久性。高原和凹槽地板提出了不同的线索，促进差异运动和变量与地形特征的相互作用。在平台表面上的SC倾向于与边缘地形有持续的相互作用，而在凹槽地板上的SC倾向于与角落和墙壁不经常接触。我们的观察表明，SC的能力进行指导，而不连续接触地形线索。SC表现出一系列不同的能动形态，其特征在于它们的对称性和数量的扩展。在所有条件下，SC与一个单一的延伸旅行显着快于细胞与更多或没有扩展。我们的结论是，SC运动是复杂的，持续的运动需要细胞的不对称性，各向异性地形与细胞规模的功能可以直接SC运动。
Directed migration of Schwann cells (SC) is critical for development and repair of the peripheral nervous system. Understanding aspects of motility specific to SC, along with SC response to engineered biomaterials, may inform strategies to enhance nerve regeneration. Rat SC were cultured on laminin-coated microgrooved poly(dimethyl siloxane) platforms that were flat or presented repeating cellular scale anisotropic topographical cues, 30 or 60 µm in width, and observed with timelapse microscopy. SC motion was directed parallel to the long axis of the topography on both the groove floor and the plateau, with accompanying differences in velocity and directional persistence in comparison to SC motion on flat substrates. In addition, feature dimension affected SC morphology, alignment, and directional persistence. Plateaus and groove floors presented distinct cues which promoted differential motility and variable interaction with the topographical features. SC on the plateau surfaces tended to have persistent interactions with the edge topography, while SC on the groove floors tended to have infrequent contact with the corners and walls. Our observations suggest the capacity of SC to be guided without continuous contact with a topographical cue. SC exhibited a range of distinct motile morphologies, characterized by their symmetry and number of extensions. Across all conditions, SC with a single extension traveled significantly faster than cells with more or no extensions. We conclude that SC motility is complex, where persistent motion requires cellular asymmetry, and that anisotropic topography with cellular scale features can direct SC motility.
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影响因子：
3.7
作者：
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