Epizootic emergence of Usutu virus in wild and captive birds in Germany.
Epizootic emergence of Usutu virus in wild and captive birds in Germany.
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DOI：
10.1371/journal.pone.0032604
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Schmidt-Chanasit J
中科院分区：
文献类型：
--
作者：
Becker N;Jöst H;Ziegler U;Eiden M;Höper D;Emmerich P;Fichet-Calvet E;Ehichioya DU;Czajka C;Gabriel M;Hoffmann B;Beer M;Tenner-Racz K;Racz P;Günther S;Wink M;Bosch S;Konrad A;Pfeffer M;Groschup MH;Schmidt-Chanasit J
这项研究旨在确定德国西南部野生和圈养鸟类大规模死亡的原因，并收集对该病原体的系统发育关系和空间分布的见解。自2011年6月以来，共收集了223只死亡禽鸟，并进行了病毒病原体检测。用实时荧光定量RT-PCR方法检测了6种86只禽类中的USUV病毒RNA。从18只黑鸟(Turdus Merula)的心脏细胞培养中分离到该病毒。免疫组织化学方法检测感染黑鸟的脑、心、肝、肺组织中USUV抗原的表达。对来自曼海姆的一只死亡黑鸟(BH65/11-02-03)的肝脏和脾样本进行了深度测序，获得了完整的多蛋白编码序列。对德国USUV毒株BH65/11-02-03的系统发育分析表明，它与2001年在奥地利造成鸟类大规模死亡的维也纳毒株有密切关系。来自德国西南部低地河谷的野生鸟类主要受到USUV的影响，但也有饲养在鸟舍中的鸟类。我们的数据表明，在2010年首次在德国蚊子中检测到USUV后，该病毒于2011年传播，并在德国西南部的野生和圈养鸟类中引发了流行性疫情。数据还表明，德国人类感染USUV的风险增加。
This study aimed to identify the causative agent of mass mortality in wild and captive birds in southwest Germany and to gather insights into the phylogenetic relationship and spatial distribution of the pathogen. Since June 2011, 223 dead birds were collected and tested for the presence of viral pathogens. Usutu virus (USUV) RNA was detected by real-time RT-PCR in 86 birds representing 6 species. The virus was isolated in cell culture from the heart of 18 Blackbirds (Turdus merula). USUV-specific antigen was demonstrated by immunohistochemistry in brain, heart, liver, and lung of infected Blackbirds. The complete polyprotein coding sequence was obtained by deep sequencing of liver and spleen samples of a dead Blackbird from Mannheim (BH65/11-02-03). Phylogenetic analysis of the German USUV strain BH65/11-02-03 revealed a close relationship with strain Vienna that caused mass mortality among birds in Austria in 2001. Wild birds from lowland river valleys in southwest Germany were mainly affected by USUV, but also birds kept in aviaries. Our data suggest that after the initial detection of USUV in German mosquitoes in 2010, the virus spread in 2011 and caused epizootics among wild and captive birds in southwest Germany. The data also indicate an increased risk of USUV infections in humans in Germany.
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影响因子：
11.8
作者：
Weissenböck H;Kolodziejek J;Url A;Lussy H;Rebel-Bauder B;Nowotny N
通讯作者：
Nowotny N
影响因子：
3.8
作者：
Buckley, A;Dawson, A;Gould, EA
通讯作者：
Gould, EA
影响因子：
5.8
作者：
Weissenböck, H;Kolodziejek, J;Nowotny, N
通讯作者：
Nowotny, N
影响因子：
9.4
作者：
Chao, Day-Yu;Davis, Brent S.;Chang, Gwong-Jen J.
通讯作者：
Chang, Gwong-Jen J.
影响因子：
2.1
作者：
Seidowski, Diana;Ziegler, Ute;Groschup, Martin H.
通讯作者：
Groschup, Martin H.