UPLC-Orbitrap-MS/MS Combined With Biochemical Analysis to Determine the Growth and Development of Mothers and Fetuses in Different Gestation Periods on Tibetan Sow Model.
UPLC-Orbitrap-MS/MS Combined With Biochemical Analysis to Determine the Growth and Development of Mothers and Fetuses in Different Gestation Periods on Tibetan Sow Model.
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UPLC-Orbitrap-MS/MS结合生化分析测定西藏母猪模型不同妊娠期母体和胎儿的生长发育
DOI：
10.3389/fnut.2022.836938
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发表时间：
2022
影响因子：
5
通讯作者：
Deng, Jinping
中科院分区：
文献类型：
--
作者：
Zhang, Longmiao;Tan, Chengquan;Xin, Zhongquan;Huang, Shuangbo;Ma, Junwu;Zhang, Meiyu;Shu, Gang;Luo, Hefeng;Deng, Baichuan;Jiang, Qingyan;Deng, Jinping
妊娠是一个复杂的动态过程，不同的妊娠阶段会影响母体的生理和代谢变化，但关于妊娠期间这些变化及其可能机制的信息很少，尤其是在血液和羊水中。本研究采用藏母猪模型，分析了不同妊娠期母体血清和羊水中生理激素和营养物质的代谢特征及其相互关系，探讨了藏母猪不同妊娠期的代谢规律。结果表明，羊水中葡萄糖、胰岛素和肝细胞生长因子浓度随妊娠的进展而降低（P < 0.05），而母血中葡萄糖和胰岛素浓度在妊娠75天时最高（P < 0.05），胰岛素与柠檬酸呈显著正相关（P < 0.05）。T4和皮质醇水平在妊娠晚期最高（P < 0.05）。此外，代谢组学分析显示，随着妊娠的进展，柠檬酸循环途径和苯丙氨酸/酪氨酸/色氨酸生物合成途径显著富集（P < 0.05）。本研究阐明了藏母猪妊娠期葡萄糖、胰岛素、柠檬酸的适应性变化以及芳香族氨基酸、肝细胞生长因子、皮质醇等生理指标对胎儿生长发育的影响，为母胎的正常发育提供了新的线索，有望成为改善妊娠期幸福感的靶点。
Pregnancy is a complex and dynamic process, the physiological and metabolite changes of the mother are affected by different pregnancy stages, but little information is available about their changes and potential mechanisms during pregnancy, especially in blood and amniotic fluid. Here, the maternal metabolism rules at different pregnancy stages were investigated by using a Tibetan sow model to analyze the physiological hormones and nutrient metabolism characteristics of maternal serum and amniotic fluid as well as their correlations with each other. Our results showed that amniotic fluid had a decrease (P < 0.05) in the concentrations of glucose, insulin and hepatocyte growth factor as pregnancy progressed, while maternal serum exhibited the highest concentrations of glucose and insulin at 75 days of gestation (P < 0.05), and a significant positive correlation (P < 0.05) between insulin and citric acid. Additionally, T4 and cortisol had the highest levels during late gestation (P < 0.05). Furthermore, metabolomics analysis revealed significant enrichment in the citrate cycle pathway and the phenylalanine/tyrosine/tryptophan biosynthesis pathway (P < 0.05) with the progress of gestation. This study clarified the adaptive changes of glucose, insulin and citric acid in Tibetan sows during pregnancy as well as the influence of aromatic amino acids, hepatocyte growth factor, cortisol and other physiological indicators on fetal growth and development, providing new clues for the normal development of the mother and the fetus, which may become a promising target for improving the well-being of pregnancy.
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