Subnuclear development of the zebrafish habenular nuclei requires ER translocon function.
Subnuclear development of the zebrafish habenular nuclei requires ER translocon function.
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DOI：
10.1016/j.ydbio.2011.09.003
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发表时间：
2011-12-01
影响因子：
2.7
通讯作者：
Gamse JT
中科院分区：
文献类型：
--
作者：
Doll CA;Burkart JT;Hope KD;Halpern ME;Gamse JT
斑马鱼背侧缰核（Dh）的特点是在基因表达，解剖和连接的显着的左，右差异。值得注意的是，Dh的外侧亚核（LsDh）在大脑左侧比右侧大，而内侧亚核（MsDh）在右侧比左侧大。筛选影响缰偏侧性的突变导致了sec 61 a样1（sec 61 al 1）基因的鉴定。在sec 61 al 1c 163突变体中，更多的LsDh神经元和更少的MsDh神经元在大脑两侧发育。LsDh中神经元的产生在突变体中比在WT中发生得更快并且持续更长的时间段。在突变体中，胚胎左侧的Nodal途径基因的表达不受影响，因为在WT胚胎的LsDh中促进神经发生的会阴旁器官的左侧放置也不受影响。上丘脑的超微结构分析表明，在WT胚胎中形成上皮的心室前体细胞在sec 61 al 1 c163突变体中失去了顶部-基底极性。我们的研究结果表明，在没有sec 61 al 1，过量的前体细胞的LsDh退出心室区和分化，导致形成双侧对称的缰核。
The dorsal habenular nuclei (Dh) of the zebrafish are characterized by significant left-right differences in gene expression, anatomy, and connectivity. Notably, the lateral subnucleus of the Dh (LsDh) is larger on the left side of the brain than on the right, while the medial subnucleus (MsDh) is larger on the right compared to the left. A screen for mutations that affect habenular laterality led to the identification of the sec61a-like 1(sec61al1) gene. In sec61al1c163 mutants, more neurons in the LsDh and fewer in the MsDh develop on both sides of the brain. Generation of neurons in the LsDh occurs more rapidly and continues for a longer time period in mutants than in WT. Expression of Nodal pathway genes on the left side of the embryos is unaffected in mutants, as is the left sided placement of the parapineal organ, which promotes neurogenesis in the LsDh of WT embryos. Ultrastructural analysis of the epithalamus indicates that ventricular precursor cells, which form an epithelium in WT embryos, lose apical-basal polarity in sec61al1c163 mutants. Our results show that in the absence of sec61al1, an excess of precursor cells for the LsDh exit the ventricular region and differentiate, resulting in formation of bilaterally symmetric habenular nuclei.
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影响因子：
16.2
作者：
Bultje, Ronald S.;Castaneda-Castellanos, David R.;Jan, Lily Yen;Jan, Yuh-Nung;Kriegstein, Arnold R.;Shi, Song-Hai
通讯作者：
Shi, Song-Hai
影响因子：
30.8
作者：
Boyadjiev, Simeon A.;Fromme, J. Christopher;Eyaid, Wafaa
通讯作者：
Eyaid, Wafaa
影响因子：
19
作者：
D'Souza-Schorey, C
通讯作者：
D'Souza-Schorey, C
影响因子：
4.6
作者：
Gamse, JT;Kuan, YS;Halpern, ME
通讯作者：
Halpern, ME
影响因子：
7.5
作者：
Farkas, Lilla M.;Huttner, Wieland B.
通讯作者：
Huttner, Wieland B.