Genomic evolution of the pathogenic Wolbachia strain, wMelPop.
Genomic evolution of the pathogenic Wolbachia strain, wMelPop.
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DOI：
10.1093/gbe/evt169
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发表时间：
2013
影响因子：
3.3
通讯作者：
O'Neill SL
中科院分区：
文献类型：
--
作者：
Woolfit M;Iturbe-Ormaetxe I;Brownlie JC;Walker T;Riegler M;Seleznev A;Popovici J;Rancès E;Wee BA;Pavlides J;Sullivan MJ;Beatson SA;Lane A;Sidhu M;McMeniman CJ;McGraw EA;O'Neill SL
广泛分布的内共生细菌Wolbachia pipientis的大多数菌株是良性的或表现为生殖寄生虫。然而，致病菌株wMelPop是一个惊人的例外：它在其昆虫宿主中过度复制，并导致严重的寿命缩短。这种发病机制目前尚不清楚。我们已经测序了wMelPop和密切相关的非致病性菌株wMelCS的三个变体的基因组。我们发现，wMelCS和wMelPop的基因组似乎是相同的基因组的非重复区域和不同的检测只有三倍的19 kb的区域，这是不太可能与寿命缩短，表明这种内共生体引起的宿主表型的显着差异可能是只有轻微的遗传变化的结果。我们还比较了来自黑腹果蝇的原始wMelPop菌株和两个连续衍生物wMelPop-CLA和wMelPop-PGYP的基因组。为了将wMelPop开发为新型生物防治剂，首先将其转染到蚊子细胞系中并在蚊子细胞系中传代约3.5年，产生wMelPop-CLA。然后将该细胞系传代的菌株转染到埃及伊蚊中，产生wMelPop-PGYP，其在昆虫宿主中4年后进行测序。我们观察到细胞系传代过程中基因组变化的快速爆发，但在转感染蚊子后没有检测到进一步的突变，这表明细胞系中发生了宿主预适应，细胞系是一个比动物宿主更具选择性的环境，或两者兼而有之。我们的研究结果提供了有价值的数据，在沃尔巴克氏体的基因组和表型变化率与主机在短时间尺度的转变。
Most strains of the widespread endosymbiotic bacterium Wolbachia pipientis are benign or behave as reproductive parasites. The pathogenic strain wMelPop is a striking exception, however: it overreplicates in its insect hosts and causes severe life shortening. The mechanism of this pathogenesis is currently unknown. We have sequenced the genomes of three variants of wMelPop and of the closely related nonpathogenic strain wMelCS. We show that the genomes of wMelCS and wMelPop appear to be identical in the nonrepeat regions of the genome and differ detectably only by the triplication of a 19-kb region that is unlikely to be associated with life shortening, demonstrating that dramatic differences in the host phenotype caused by this endosymbiont may be the result of only minor genetic changes. We also compare the genomes of the original wMelPop strain from Drosophila melanogaster and two sequential derivatives, wMelPop-CLA and wMelPop-PGYP. To develop wMelPop as a novel biocontrol agent, it was first transinfected into and passaged in mosquito cell lines for approximately 3.5 years, generating wMelPop-CLA. This cell line-passaged strain was then transinfected into Aedes aegypti mosquitoes, creating wMelPop-PGYP, which was sequenced after 4 years in the insect host. We observe a rapid burst of genomic changes during cell line passaging, but no further mutations were detected after transinfection into mosquitoes, indicating either that host preadaptation had occurred in cell lines, that cell lines are a more selectively permissive environment than animal hosts, or both. Our results provide valuable data on the rates of genomic and phenotypic change in Wolbachia associated with host shifts over short time scales.
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影响因子：
6.7
作者：
Andrews ES;Crain PR;Fu Y;Howe DK;Dobson SL
通讯作者：
Dobson SL
DOI：
10.1007/978-3-642-21078-5_13
发表时间：
2011-01-01
期刊：
RIBONUCLEASES
影响因子：
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作者：
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通讯作者：
Krasilnikov, Andrey S.
影响因子：
4.5
作者：
Eppinger, Mark;Baar, Claudia;Schuster, Stephan C.
通讯作者：
Schuster, Stephan C.
影响因子：
3.3
作者：
Delihas N
通讯作者：
Delihas N
DOI：
10.1007/978-0-387-78225-6_11
发表时间：
2008-01-01
期刊：
TRANSGENESIS AND THE MANAGEMENT OF VECTOR-BORNE DISEASE
影响因子：
--
作者：
Cook, Peter E.;McMeniman, Conor J.;O'Neill, Scott L.
通讯作者：
O'Neill, Scott L.