Reactive oxygen species production and Brugia pahangi survivorship in Aedes polynesiensis with artificial Wolbachia infection types.
Reactive oxygen species production and Brugia pahangi survivorship in Aedes polynesiensis with artificial Wolbachia infection types.
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DOI：
10.1371/journal.ppat.1003075
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发表时间：
2012
期刊：
影响因子：
6.7
通讯作者：
Dobson SL
中科院分区：
文献类型：
--
作者：
Andrews ES;Crain PR;Fu Y;Howe DK;Dobson SL
先前已显示内共生细菌沃尔巴克氏体的异源转感染在蚊子宿主中诱导病原体干扰表型。在这里，我们研究了人工感染的波利尼西亚伊蚊，班氏吴策线虫，这是在南太平洋大部分地区的淋巴丝虫病（LF）的病原体的主要载体菌株。采用胚胎显微注射将白纹伊蚊的wAlbB感染转移到Ae的脱共生菌株中。波利尼西亚。所得菌株（命名为“MTB”）经历具有高母体遗传的稳定人工感染。MTB与自然感染的野生型Ae.波利尼西亚种表现出强烈的双向不亲和性。MTB菌株中活性氧（ROS）的水平相对于野生型显著不同，表明调节氧化应激的能力受损。与自然感染和非共生菌株相比，在用帕汉布氏丝虫攻击后，MTB中达到感染阶段的蠕虫数量显著减少。MTB的存活率与野生型的存活率显著不同，存活率和血液喂养之间存在交互作用。结果表明，ROS水平下降和成年雌性波利尼西亚伊蚊的存活率下降之间存在直接相关性。结果进行了讨论，在相互作用的Wolbachia与ROS的生产和抗氧化剂的表达，铁稳态和昆虫免疫系统。我们讨论了潜在的应用MTB菌株的影响AE。波利尼西亚种群和减少南太平洋LF发病率的战略。淋巴丝虫病（LF）是南太平洋地区发病的主要原因，由丝虫班氏吴策线虫引起。在南太平洋消除LF需要一种方法，将大规模药物管理和针对主要蚊子媒介波利尼西亚伊蚊的战略结合起来。AE.波利尼西亚蚊子自然感染沃尔巴克氏体，沃尔巴克氏体是一种内共生细菌，由于其影响蚊子繁殖和干扰病原体发育的能力，是新的控制策略的焦点。人工Wolbachia感染与活性氧（ROS）水平增加有关，这可以改变免疫基因表达并抑制登革热增殖。在这里，我们描述了Ae的生成。polynesiensis菌株，该菌株已被人工感染来自Ae.白纹伊蚊感染是稳定的，并导致条件性不育时，与野生型杂交。人工感染的菌株表现出与野生型不同的ROS水平，表明调节氧化应激的能力降低。在人工感染的品系中，成功发育成感染期蠕虫的数量减少。此外，人工感染菌株的存活率显著低于野生型。人工感染的Ae。波利尼西亚菌株进行了讨论有关正在进行的蚊媒疾病控制工作。
Heterologous transinfection with the endosymbiotic bacterium Wolbachia has been shown previously to induce pathogen interference phenotypes in mosquito hosts. Here we examine an artificially infected strain of Aedes polynesiensis, the primary vector of Wuchereria bancrofti, which is the causative agent of Lymphatic filariasis (LF) throughout much of the South Pacific. Embryonic microinjection was used to transfer the wAlbB infection from Aedes albopictus into an aposymbiotic strain of Ae. polynesiensis. The resulting strain (designated “MTB”) experiences a stable artificial infection with high maternal inheritance. Reciprocal crosses of MTB with naturally infected wild-type Ae. polynesiensis demonstrate strong bidirectional incompatibility. Levels of reactive oxygen species (ROS) in the MTB strain differ significantly relative to that of the wild-type, indicating an impaired ability to regulate oxidative stress. Following a challenge with Brugia pahangi, the number of filarial worms achieving the infective stage is significantly reduced in MTB as compared to the naturally infected and aposymbiotic strains. Survivorship of MTB differed significantly from that of the wild-type, with an interactive effect between survivorship and blood feeding. The results demonstrate a direct correlation between decreased ROS levels and decreased survival of adult female Aedes polynesiensis. The results are discussed in relation to the interaction of Wolbachia with ROS production and antioxidant expression, iron homeostasis and the insect immune system. We discuss the potential applied use of the MTB strain for impacting Ae. polynesiensis populations and strategies for reducing LF incidence in the South Pacific. Lymphatic filariasis (LF), the leading cause of morbidity in South Pacific regions, is caused by a filarial worm, Wuchereria bancrofti. Elimination of LF in the South Pacific requires an approach integrating both mass drug administration and strategies targeting the primary mosquito vector, Aedes polynesiensis. Ae. polynesiensis is naturally infected with Wolbachia, an endosymbiotic bacterium that is a focus of novel control strategies, due to its ability to affect mosquito reproduction and interfere with pathogen development. Artificial Wolbachia infections are associated with increased levels of reactive oxygen species (ROS), which can alter immune gene expression and inhibit dengue proliferation. Here, we describe the generation of an Ae. polynesiensis strain that has been artificially infected with Wolbachia from Ae. albopictus. The infection is stably maintained and causes conditional sterility when crossed with the wild-type. The artificially infected strain exhibits different ROS levels than the wild-type, indicating a decreased ability to regulate oxidative stress. The number of successfully developing infective stage filarial worms was reduced in the artificially infected strain. In addition, survival of the artificially infected strain was significantly lower than the wild-type. The artificially infected Ae. polynesiensis strain is discussed in relation to ongoing mosquito-borne disease control efforts.
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影响因子：
2.1
作者：
Brelsfoard, C. L.;Dobson, S. L.
通讯作者：
Dobson, S. L.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.7
作者：
Brennan LJ;Keddie BA;Braig HR;Harris HL
通讯作者：
Harris HL
影响因子：
3.7
作者：
Gonçalves RL;Machado AC;Paiva-Silva GO;Sorgine MH;Momoli MM;Oliveira JH;Vannier-Santos MA;Galina A;Oliveira PL;Oliveira MF
通讯作者：
Oliveira MF
影响因子：
2.6
作者：
Dutton, TJ;Sinkins, SP
通讯作者：
Sinkins, SP