High level of phosphatidylcholines/lysophosphatidylcholine ratio in urine is associated with prostate cancer.
High level of phosphatidylcholines/lysophosphatidylcholine ratio in urine is associated with prostate cancer.
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DOI：
10.1111/cas.15093
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发表时间：
2021-10
期刊：
影响因子：
5.7
通讯作者：
Inoue T
中科院分区：
文献类型：
--
作者：
Li X;Nakayama K;Goto T;Kimura H;Akamatsu S;Hayashi Y;Fujita K;Kobayashi T;Shimizu K;Nonomura N;Ogawa O;Inoue T
在我们之前的研究结果中，前列腺癌（CaP）和良性组织中磷脂（PL）和溶血磷脂（LPL）水平的改变促使我们探索PL和LPL作为CaP的潜在生物标志物。尿脂质组学在前列腺癌的临床诊断和治疗中越来越受到重视。在这项研究中，使用高分辨率基质辅助激光解吸/电离成像质谱（HR‐MALDI‐IMS）评估了31例从根治性前列腺切除术中获得的前列腺组织。在直肠指检（DRE）后采集尿样，并使用酸化Bligh-Dyer法提取尿脂质。发现集包括京都大学医院的75例CaP患者和44例良性前列腺增生（BPH）患者;验证集包括大坂大学医院的74例CaP患者和59例BPH患者。使用MALDI飞行时间MS（MALDI-TOF/MS）进行尿脂质组学筛查。比较CaP组和BPH组尿溶血磷脂酰胆碱（LPC）和磷脂酰胆碱（PC）水平。CaP组织中的（PC [34：2] + PC [34：1]）/LPC（16：0）比显著高于良性上皮组织（P <0.001）。在发现集和验证集中，CaP组的尿PC/LPC比值显著高于BPH组（P <0.001）。京都大学医院（KUHP）/发现集和大坂大学医院（OUHP）/验证集中尿磷脂酰胆碱/溶血磷脂酰胆碱（PC/LPC）比值和血清前列腺特异性抗原（PSA）水平的散点图。
The altered levels of phospholipids (PLs) and lysophospholipids (LPLs) in prostate cancer (CaP) and benign tissues in our previous findings prompted us to explore PLs and LPLs as potential biomarkers for CaP. Urinary lipidomics has attracted increasing attention in clinical diagnostics and prognostics for CaP. In this study, 31 prostate tissues obtained from radical prostatectomy were assessed using high‐resolution matrix‐assisted laser desorption/ionization imaging mass spectrometry (HR‐MALDI‐IMS). Urine samples were collected after digital rectal examination (DRE), and urinary lipids were extracted using the acidified Bligh‐Dyer method. The discovery set comprised 75 patients with CaP and 44 with benign prostatic hyperplasia (BPH) at Kyoto University Hospital; the validation set comprised 74 patients with CaP and 59 with BPH at Osaka University Hospital. Urinary lipidomic screening was performed using MALDI time‐of‐flight MS (MALDI‐TOF/MS). The levels of urinary lysophosphatidylcholine (LPC) and phosphatidylcholines (PCs) were compared between the CaP and BPH groups. The (PC [34:2] + PC [34:1])/LPC (16:0) ratio was significantly higher (P < .001) in CaP tissues than in benign epithelial tissues. The urinary PCs/LPC ratio was significantly higher (P < .001) in the CaP group than in the BPH group in the discovery and validation sets. Scatter plots of the urinary phosphatidylcholines/lysophosphatidylcholine (PCs/LPC) ratios and serum prostate‐specific antigen (PSA) levels in the Kyoto University Hospital (KUHP)/discovery and Osaka University Hospital (OUHP)/validation sets.
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影响因子：
3.7
作者：
Patel N;Vogel R;Chandra-Kuntal K;Glasgow W;Kelavkar U
通讯作者：
Kelavkar U
影响因子：
3.9
作者：
Awwad, Hussain Mohamad;Ohlmann, Carsten-Henning;Obeid, Rima
通讯作者：
Obeid, Rima
影响因子：
6.6
作者：
Faas, FH;Dang, AQ;Johnson, D
通讯作者：
Johnson, D
影响因子：
4.3
作者：
Jeong, Rae Ung;Lim, Sangsoo;Moon, Myeong Hee
通讯作者：
Moon, Myeong Hee
影响因子：
5.1
作者：
Menschikowski, Mario;Hagelgans, Albert;Siegert, Gabriele
通讯作者：
Siegert, Gabriele