Effects of Surface Nanotopography and Calcium Chemistry of Titanium Bone Implants on Early Blood Platelet and Macrophage Cell Function.
Effects of Surface Nanotopography and Calcium Chemistry of Titanium Bone Implants on Early Blood Platelet and Macrophage Cell Function.
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DOI：
10.1155/2018/1362958
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发表时间：
2018
影响因子：
--
通讯作者：
Hanawa T
中科院分区：
文献类型：
--
作者：
Park JW;Han SH;Hanawa T
血小板和免疫炎症细胞（巨噬细胞）对钛（Ti）骨植入物的早期反应，通过形成临时纤维蛋白基质支架，促进干细胞迁移和生长因子和细胞因子的产生，调节早期组织愈合微环境，影响植入物随后的生物愈合。本研究探讨了纳米级表面形貌和钛表面钙离子（Ca2+）修饰对血小板和巨噬细胞调节的生物相容性的影响，为未来钛骨植入物的表面设计提供了增强早期成骨能力的基础。采用水热法制备了Ca2+富集或不富集的纳米钛表面。通过形态学观察血小板扩散和纤维蛋白基质形成、血小板生长因子释放、巨噬细胞表型免疫染色和巨噬细胞炎性细胞因子产生，研究改性钛表面对血小板和巨噬细胞的即时和早期功能的调节。结果表明，钛的表面纳米级形貌修饰可促进血小板活化，抑制巨噬细胞的炎症反应。此外，Ca2+表面化学修饰增强了纳米结构Ti表面的血小板响应调节功能，加速了纤维蛋白基质的即时形成和血小板源性生长因子- ab的释放。因此，种植体表面的纳米形貌和Ca2+修饰有望通过积极调节即时血小板功能和早期巨噬细胞免疫炎症反应来促进钛骨种植体周围伤口愈合的初始阶段。
Early responses of blood platelets and immunoinflammatory cells (macrophages) to titanium (Ti) bone implants affect the subsequent biological healing of implants by modulating early tissue healing-microenvironments via the formation of temporary fibrin matrix scaffolds for stem cell migration and production of growth factors and cytokines. This study investigated the effects of nanoscale surface topography and calcium ion (Ca2+) modification of Ti surfaces on biocompatibility regulated by blood platelets and macrophages, for the future surface design of Ti bone implants with enhanced early osteogenic capacity. A nanostructured Ti surface with or without Ca2+ enrichment was prepared using the hydrothermal treatment. Immediate and early functions of platelets and macrophages modulated by modified Ti surfaces were investigated by morphological observation of platelet spreading and fibrin matrix formation, platelet growth factor release, immunostaining of macrophage phenotypes, and macrophage inflammatory cytokine production. The results showed that surface nanoscale topographical modification of Ti promotes blood platelet activation and suppresses the inflammatory response of macrophages. In addition, surface chemistry modifications with Ca2+ enhanced the platelet response-modulating function of the nanostructured Ti surface, which accelerated immediate fibrin matrix formation and platelet-derived growth factor-AB release. Thus, nanotopographical and Ca2+ modifications of implant surfaces are expected to be effective approaches that favor the initial phase of wound healing around the Ti bone implants through positive modulation of immediate blood platelet function and early macrophage immunoinflammatory response.
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影响因子：
5.8
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