Extrauterine listeriosis in the gravid mouse influences embryonic growth and development.
Extrauterine listeriosis in the gravid mouse influences embryonic growth and development.
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DOI：
10.1371/journal.pone.0072601
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Orndorff PE
中科院分区：
文献类型：
--
作者：
Suyemoto MM;Hamrick TS;Spears PA;Horton JR;Washington IM;Havell EA;Borst LB;Orndorff PE
接种单核细胞增生李斯特氏菌的怀孕小鼠和其他啮齿动物通常无法清除子宫内感染，并且会死亡或排出其宫内内容物。我们利用这一特性来研究宫外感染对妊娠成功参数的影响。如果怀孕小鼠在妊娠 7.5 天 (gd) 口服接种后没有表现出李斯特氏菌病的临床症状，并且在近期 (18.5 gd) 时没有可检测到的宫内菌落形成单位 (cfu)，则选择它们进行我们的研究。对于两种李斯特菌血清型菌株（4nonb 和 1/2a），所采用的口服剂量范围为每只小鼠 106-108 cfu。与假接种对照相比，在所有剂量下，接种均导致每窝平均近期胎儿体重（18.5 gd）下降。此外，一些接种的小鼠表现出胚胎死亡（由宫内吸收表明），但在所有假接种的动物中均不存在。在旨在检测可能的胎盘功能丧失的平行实验中，在口服接种后 96 小时对妊娠子宫进行组织病理学和微生物学检查。胎盘病变与高 (> 106) 宫内 cfu 相关，但与低 (< 102) 或缺失宫内 cfu 无关。在体外，小鼠胚胎滋养层细胞与小鼠肠上皮细胞对李斯特菌暴露的敏感性没有区别。与我们的观察一致的模型是急性感染期间产生的产物（宿主或细菌）经胎盘进入胚胎并影响胚胎存活并减缓子宫内的正常生长。
Gravid mice and other rodents inoculated with Listeria monocytogenes typically fail to clear an intrauterine infection and either succumb or expel their intrauterine contents. We took advantage of this property to investigate the effects of an extrauterine infection on parameters of pregnancy success. Pregnant mice were selected for our study if they showed no clinical signs of listeriosis following oral inoculation at 7.5 gestational days (gd), and had no detectable intrauterine colony forming units (cfu) at near term (18.5 gd). The range of oral doses employed was 106-108 cfu per mouse for two listerial serotype strains (4nonb and 1/2a). At all doses, inoculation resulted in a decrease in average near-term (18.5 gd) fetal weight per litter compared to sham inoculated controls. Additionally, embryonic death (indicated by intrauterine resorptions) was exhibited by some inoculated mice but was absent in all sham inoculated animals. In parallel experiments designed to detect possible loss of placental function, gravid uteruses were examined histopathologically and microbiologically 96 h after oral inoculation. Placental lesions were associated with high (> 106), but not low (< 102) or absent intrauterine cfu. In vitro, mouse embryonic trophoblasts were indistinguishable from mouse enterocytes in terms of their sensitivity to listerial exposure. A model consistent with our observations is one in which products (host or bacterial) generated during an acute infection enter embryos transplacentally and influences embryonic survival and slows normal growth in utero.
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影响因子：
6.7
作者：
Rowe JH;Ertelt JM;Xin L;Way SS
通讯作者：
Way SS
影响因子：
3.1
作者：
PINE, L;MALCOLM, GB;PLIKAYTIS, BD
通讯作者：
PLIKAYTIS, BD
影响因子：
64.5
作者：
Wollert, Thomas;Pasche, Bastian;Schubert, Wolf-Dieter
通讯作者：
Schubert, Wolf-Dieter
影响因子：
3.6
作者：
Bowen, JA;Hunt, JS
通讯作者：
Hunt, JS
影响因子：
2.2
作者：
VIDAL, K;GROSJEAN, I;KAISERLIAN, D
通讯作者：
KAISERLIAN, D