A genetic approach to the development of new therapeutic phages to fight pseudomonas aeruginosa in wound infections.
A genetic approach to the development of new therapeutic phages to fight pseudomonas aeruginosa in wound infections.
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DOI：
10.3390/v5010015
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发表时间：
2012-12-21
期刊：
影响因子：
--
通讯作者：
Pleteneva E
中科院分区：
文献类型：
--
作者：
Krylov V;Shaburova O;Krylov S;Pleteneva E
铜绿假单胞菌是伤口感染的常见参与者。多种抗生素抗性菌株的出现在感染伤口的治疗中产生了重大问题。噬菌体治疗（PT）已被提出作为一种可能的替代方法。感染伤口是PT应用的理想场所，因为PT的基本条件得到了保证;即细菌及其病毒的直接接触。在铜绿假单胞菌中已知大量的毒性（“裂解性”）和温和性（“溶原性”）噬菌体。然而，PT可接受的毒性噬菌体种类的数量及其突变性是有限的。此外，有毒（和温和）的行为有不同的偏差，从他们预期的典型模式的发展。我们考虑了一些非典型的噬菌体-细菌相互作用的例子和它们在PT中使用的可能性。此外，将从生物学家的角度讨论噬菌体治疗发展的一些最佳方法，考虑到噬菌体辅助水平基因转移（HGT）的危险，并从接受希波克拉底誓言的外科医生的角度讨论通过一切可能的手段治愈患者。现在也是时候讨论国际合作促进PT发展的可能方法了。我们认为，使噬菌体治疗成为一种个性化的药物是有利的。
Pseudomonas aeruginosa is a frequent participant in wound infections. Emergence of multiple antibiotic resistant strains has created significant problems in the treatment of infected wounds. Phage therapy (PT) has been proposed as a possible alternative approach. Infected wounds are the perfect place for PT applications, since the basic condition for PT is ensured; namely, the direct contact of bacteria and their viruses. Plenty of virulent (“lytic”) and temperate (“lysogenic”) bacteriophages are known in P. aeruginosa. However, the number of virulent phage species acceptable for PT and their mutability are limited. Besides, there are different deviations in the behavior of virulent (and temperate) phages from their expected canonical models of development. We consider some examples of non-canonical phage-bacterium interactions and the possibility of their use in PT. In addition, some optimal approaches to the development of phage therapy will be discussed from the point of view of a biologist, considering the danger of phage-assisted horizontal gene transfer (HGT), and from the point of view of a surgeon who has accepted the Hippocrates Oath to cure patients by all possible means. It is also time now to discuss the possible approaches in international cooperation for the development of PT. We think it would be advantageous to make phage therapy a kind of personalized medicine.
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影响因子：
3.5
作者：
Byrne, M;Kropinski, AM
通讯作者：
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影响因子：
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影响因子：
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