Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential.
Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential.
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DOI：
10.1186/1472-6750-11-94
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发表时间：
2011-10-13
影响因子：
3.5
通讯作者：
Ruijssenaars HJ
中科院分区：
文献类型：
--
作者：
Bandounas L;Wierckx NJ;de Winde JH;Ruijssenaars HJ
为了扩大从木质纤维素生物质中获得的产品范围，木质素成分应用作替代芳烃等增值化学品的原料，而不是通过焚烧来获取热量和能源。酶可以为木质素解聚成感兴趣的产品提供有效的手段。在这项研究中，通过富集硫酸盐木质素来分离土壤细菌，并评估其作为废木质素增值新型酶来源的木质素分解潜力。基于16S rRNA基因测序和表型表征，该生物体被鉴定为Pandoraea norimbergensis LD001、Pseudomonas sp LD002和Bacillus sp LD003。通过在高分子量和低分子量木质素级分上的生长、木质素相关芳香族单体的利用以及木质素分解指示剂染料的降解来评估这些分离物中的每一个的木质素分解能力。 Pandoraea norimbergensis LD001 和假单胞菌 sp. LD002 在木质素级分上表现出最佳生长，但染料脱色能力有限。芽孢杆菌属。然而，LD003 在木质素馏分上的生长效率最低，但具有广泛的染料脱色能力，特别偏向顽固的吩噻嗪染料类（天青 B、亚甲蓝和甲苯蓝 O）。芽孢杆菌属。 LD003 被选为新型木质素分解酶的有前途的来源。我们的观察表明，木质素矿化和解聚是独立的事件，这给筛选木质素微生物中的特定木质素分解酶带来了额外的挑战。
To expand on the range of products which can be obtained from lignocellulosic biomass, the lignin component should be utilized as feedstock for value-added chemicals such as substituted aromatics, instead of being incinerated for heat and energy. Enzymes could provide an effective means for lignin depolymerization into products of interest. In this study, soil bacteria were isolated by enrichment on Kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for waste lignin valorization. Based on 16S rRNA gene sequencing and phenotypic characterization, the organisms were identified as Pandoraea norimbergensis LD001, Pseudomonas sp LD002 and Bacillus sp LD003. The ligninolytic capability of each of these isolates was assessed by growth on high-molecular weight and low-molecular weight lignin fractions, utilization of lignin-associated aromatic monomers and degradation of ligninolytic indicator dyes. Pandoraea norimbergensis LD001 and Pseudomonas sp. LD002 exhibited best growth on lignin fractions, but limited dye-decolourizing capacity. Bacillus sp. LD003, however, showed least efficient growth on lignin fractions but extensive dye-decolourizing capacity, with a particular preference for the recalcitrant phenothiazine dye class (Azure B, Methylene Blue and Toluidene Blue O). Bacillus sp. LD003 was selected as a promising source of novel types of ligninolytic enzymes. Our observations suggested that lignin mineralization and depolymerization are separate events which place additional challenges on the screening of ligninolytic microorganisms for specific ligninolytic enzymes.
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影响因子：
4.4
作者：
ARCHIBALD, FS
通讯作者：
ARCHIBALD, FS
DOI：
10.1073/pnas.0805257105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Geib, Scott M.;Filley, Timothy R.;Tien, Ming
通讯作者：
Tien, Ming
影响因子：
11.4
作者：
Emmel, A;Mathias, AL;Ramos, LP
通讯作者：
Ramos, LP
DOI：
10.1099/00207713-50-2-887
发表时间：
2000-03-01
影响因子：
2.8
作者：
Coenye, T;Falsen, E;Vandamme, P
通讯作者：
Vandamme, P
影响因子：
13.6
作者：
Banerjee, Aditi;Ghoshal, Aloke K.
通讯作者：
Ghoshal, Aloke K.