Tissue-Specific Near-Infrared Fluorescence Imaging.
Tissue-Specific Near-Infrared Fluorescence Imaging.
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组织特异性近红外荧光成像。
DOI：
10.1021/acs.accounts.6b00239
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发表时间：
2016-09-20
影响因子：
18.3
通讯作者：
Choi HS
中科院分区：
文献类型：
--
作者：
Owens EA;Henary M;El Fakhri G;Choi HS
近红外(NIR)荧光光通过为外科医生提供目标组织的高度特异性图像，在临床成像中得到了广泛的应用。从650到900 nm的“近红外窗口”特别有用，因为它有几个特殊的特点，例如组织中生物分子的最小自发荧光和吸收，以及低光散射。与可见光相比，近红外荧光光是不可见的，因此可以在不改变手术视野的情况下，在人类手术中进行高灵敏度的实时图像引导。近红外荧光光作为一种临床影像技术的好处可以归功于它作为一种外源性造影剂的分子荧光。事实上，单光子发射计算机断层扫描(SPECT)和正电子发射断层扫描(PET)术前全身成像在诊断方面仍然很重要，但它们缺乏无害和有针对性的近红外荧光团的功效，无法在保留重要组织的同时促进病变组织的实时描绘。诚然，近红外成像技术进入临床应用的速度一直很慢，主要是因为用于当前成像系统的真正突破性造影剂的开发较晚。因此，清楚地确定肿瘤组织的物理边缘在生物成像和靶向治疗中仍然是至关重要的。另一个同样值得注意但研究较少的目标是能够勾勒出健康的重要组织，这些组织应该在术中不横切的情况下仔细导航。这两条途径都需要优化设计考虑因素的挑战，不仅要在近红外窗口中获得有效的成像剂，而且要获得高分子亮度、水溶性、生物相容性和组织特异性靶向性。成像界认识到三种战略方法，包括(1)通过EPR效应的被动靶向，(2)利用已知分子固有的整体生物分布的主动靶向，以及(3)通过内部刺激激活靶向，从关闭状态打开荧光。纳米医学和生物成像领域的最新进展为满足这些严格的要求提供了迫切需要的希望，因为我们开发了一种成功的靶向造影剂目录，通过使用光谱不同的实时荧光团来照亮同一手术空间中的肿瘤和重要组织。这些组织特异性造影剂可以作为医生的多功能武器，用于术中实时导航和图像引导的靶向治疗。文献中有一个多用途的组织特异性荧光团文库，这里讨论了许多，它为临床医生提供了一系列的可能性，无疑将改善手术中的成功率和术后的长期预后。
Near-infrared (NIR) fluorescence light has been widely utilized in clinical imaging by providing surgeons highly specific images of target tissue. The “NIR window” from 650 to 900 nm is especially useful due to several special features such as minimal autofluorescence and absorption of biomolecules in tissue, as well as low light scattering. Compared with visible wavelengths, NIR fluorescence light is invisible, thus allowing highly sensitivity real-time image guidance in human surgery without changing the surgical field. The benefit of using NIR fluorescence light as a clinical imaging technology can be attributed to its molecular fluorescence as an exogenous contrast agent. Indeed, whole body preoperative imaging of single-photon emission computed tomography (SPECT) and positron emission tomography (PET) remains important in diagnostic utility, but they lack the efficacy of innocuous and targeted NIR fluorophores to simultaneously facilitate the real-time delineation of diseased tissue while preserving vital tissues. Admittedly, NIR imaging technology has been slow to enter clinical use mostly due to the late-coming development of truly breakthrough contrast agents for use with current imaging systems. Therefore, clearly defining the physical margins of tumorous tissue remains of paramount importance in bioimaging and targeted therapy. An equally noteworthy yet less researched goal is the ability to outline healthy vital tissues that should be carefully navigated without transection during the intraoperative surgery. Both of these paths require optimizing a gauntlet of design considerations to obtain not only an effective imaging agent in the NIR window but also high molecular brightness, water solubility, biocompatibility, and tissue-specific targetability. The imaging community recognizes three strategic approaches which include (1) passive targeting via the EPR effect, (2) active targeting using the innate overall biodistribution of known molecules, and (3) activatable targeting through an internal stimulus, which turns on fluorescence from an off state. Recent advances in nanomedicine and bioimaging offer much needed promise toward fulfilling these stringent requirements as we develop a successful catalog of targeted contrast agents for illuminating both tumors and vital tissues in the same surgical space by employing spectrally distinct fluorophores in real time. These tissue-specific contrast agents can be versatile arsenals to physicians for real-time intraoperative navigation as well as image-guided targeted therapy. There is a versatile library of tissue-specific fluorophores available in the literature, with many discussed herein, which offers clinicians an array of possibilities that will undoubtedly improve intraoperative success and long-term postoperation prognosis.
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DOI：
10.1002/wnan.1337
发表时间：
2015-11
期刊：
Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology
影响因子：
--
作者：
Owens EA;Lee S;Choi J;Henary M;Choi HS
通讯作者：
Choi HS
影响因子：
12.4
作者：
Bao K;Nasr KA;Hyun H;Lee JH;Gravier J;Gibbs SL;Choi HS
通讯作者：
Choi HS
用于甲状旁腺和甲状腺成像的近红外荧光团的结构固有靶向。
影响因子：
82.9
作者：
通讯作者：
--
用于近红外癌症治疗诊断的 pH 可激活苯胺取代光敏剂
影响因子：
8.4
作者：
Tian J;Zhou J;Shen Z;Ding L;Yu JS;Ju H
通讯作者：
Ju H
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru