Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions.
Aspergillus nidulans transcription factor AtfA interacts with the MAPK SakA to regulate general stress responses, development and spore functions.
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DOI：
10.1111/j.1365-2958.2011.07581.x
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发表时间：
2011-04
影响因子：
3.6
通讯作者：
Aguirre J
中科院分区：
文献类型：
--
作者：
Lara-Rojas F;Sánchez O;Kawasaki L;Aguirre J
真菌利用与MAP激酶模块耦合的磷酸继电器系统来感知和处理环境信号。在nidulans中，反应调节子SskA向应激MAPK(SAPK)SAKA传递渗透和氧化应激信号。利用遗传方法结合GFP标记和分子双荧光，我们发现SAKA和ATF/CREB转录因子ATFA定义了一个一般的胁迫信号通路，在生长和发育过程中的氧化应激反应中扮演着不同的角色。ATFA永久地定位在细胞核中，而SAKA在响应氧化或渗透胁迫信号时或在正常的孢子发育过程中积累在细胞核中，在那里它与ATFA物理上相互作用。ATFA对于多种基因的表达、SAKA分生孢子的积累和分生孢子的存活都是必需的。此外，SAKA在无性孢子中是活跃的(磷酸化)，在休眠分生孢子中保持磷酸化，并在萌发过程中去磷酸化。孢子中SAKA的磷酸化依赖于磷传递系统的某些(SskA)而不是其他(SrA和Nika)成分。杀菌剂氟二考尼诱导SAKA的结构性磷酸化，阻止两者，胚管形成和核分裂。类似地，粗糙脉孢霉OS-2在完整的分生孢子中被磷酸化，并在萌发过程中被去磷酸化。我们认为SAKA-ATFA相互作用调节胁迫和分生孢子发育过程中的基因表达，SAPK磷酸化是调节不生长(孢子)和生长(菌丝)状态之间转换的一种保守机制。
Fungi utilize a phosphorelay system coupled to a MAP kinase module for sensing and processing environmental signals. In Aspergillus nidulans, response regulator SskA transmits osmotic and oxidative stress signals to the stress MAPK (SAPK) SakA. Using a genetic approach together with GFP tagging and molecular bifluorescence we show that SakA and ATF/CREB transcription factor AtfA define a general stress-signalling pathway that plays differential roles in oxidative stress responses during growth and development. AtfA is permanently localized in the nucleus, while SakA accumulates in the nucleus in response to oxidative or osmotic stress signals or during normal spore development, where it physically interacts with AtfA. AtfA is required for expression of several genes, the conidial accumulation of SakA and the viability of conidia. Furthermore, SakA is active (phosphorylated) in asexual spores, remaining phosphorylated in dormant conidia and becoming dephosphorylated during germination. SakA phosphorylation in spores depends on certain (SskA) but not other (SrrA and NikA) components of the phosphorelay system. Constitutive phosphorylation of SakA induced by the fungicide fludioxonil prevents both, germ tube formation and nuclear division. Similarly, Neurospora crassa SakA orthologue OS-2 is phosphorylated in intact conidia and gets dephosphorylated during germination. We propose that SakA–AtfA interaction regulates gene expression during stress and conidiophore development and that SAPK phosphorylation is a conserved mechanism to regulate transitions between non-growing (spore) and growing (mycelia) states.
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影响因子：
3.2
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