Differences between adult and adolescent male mice in approach/avoidance and expression of hippocampal NPY in response to acute footshock.
Differences between adult and adolescent male mice in approach/avoidance and expression of hippocampal NPY in response to acute footshock.
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DOI：
10.1080/10253890.2021.1976139
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发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
Dobrunz LE
中科院分区：
文献类型：
--
作者：
Cortes MA;Corder KM;Dobrunz LE
焦虑症是青春期和成年期最常见的神经精神疾病。压力会导致焦虑相关行为的增加，尽管压力对啮齿类动物的影响通常只在成年人中进行研究。患有创伤后应激障碍的成年患者中，神经肽 Y（NPY）（一种应激恢复介质）的水平降低。对于啮齿类动物来说，足部电击是一种身体压力源，会增加成年人的焦虑样行为并减少 NPY，但对青少年的影响尚不清楚。在这里，我们使用 30 分钟不可预测的足部电击方案来研究青少年（6 周）和成年（3 个月）雄性 C57Bl6/J 小鼠之间行为和压力相关分子的差异。通过足部电击期间的冰冻增强以及暴露后不久血浆皮质酮和 NPY 的升高观察到，该方案导致两个年龄段的恐惧表达。然而，两个年龄段对接近/回避行为的影响是不同的。足部电击暴露一周后，成年小鼠的张臂时间和进入高架十字迷宫的时间减少，而青少年小鼠则没有任何影响。两个年龄组的足部电击小鼠在高架十字迷宫和开放场地中的活动水平均降低。运动不足与运动缺陷无关，因为足部电击组和使用旋转棒的对照组之间没有差异。令人惊讶的是，我们发现青少年小鼠海马中的 NPY 肽表达升高，而成年小鼠在足部电击暴露一周后表达降低。总之，这些结果表明，与成人相比，压力对青少年的行为和重要的压力恢复因子 NPY 的影响存在差异。
Anxiety disorders are the most common neuropsychiatric disorders diagnosed in adolescence and adulthood. Stress can lead to an increase in anxiety-related behaviors, although the consequences of stress in rodents are typically investigated only in adults. The levels of Neuropeptide Y (NPY), a mediator of stress resilience, are reduced in adult patients with Post-Traumatic Stress Disorder. For rodents, footshock is a physical stressor that increases anxiety-like behavior and reduces NPY in adults, however the effects in adolescents are unknown. Here we used a 30-minute unpredictable footshock protocol to investigate the differences in behavior and stress-relevant molecules between adolescent (6 weeks) and adult (3 months) male C57Bl6/J mice. The protocol resulted in fear expression in both ages as observed by enhanced freezing during footshock and elevation in plasma corticosterone and NPY shortly after exposure. However, effects on approach/avoidance behavior were different between the two ages. One week after footshock exposure, adult mice showed reduced open arm time and entries on elevated plus maze, whereas adolescent mice showed no effect. Footshock mice in both age groups displayed reduced activity levels in elevated plus maze and open field. The hypolocomotion did not relate to motor deficits, as there were no differences between footshock and control groups using rotarod. Surprisingly, we found that the adolescent mice had elevated NPY peptide expression in hippocampus, whereas adults had reduced expression one week after footshock exposure. Together, these results demonstrate that stress differentially affects both behavior and the important stress resilience factor NPY in adolescents compared to adults.
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影响因子：
3.7
作者：
Corder KM;Cortes MA;Bartley AF;Lear SA;Lubin FD;Dobrunz LE
通讯作者：
Dobrunz LE
影响因子：
3.3
作者：
Eiland, L.;Romeo, R. D.
通讯作者：
Romeo, R. D.
影响因子：
2.9
作者：
Post RJ;Dahlborg KM;O'Loughlin LE;Bloom CM
通讯作者：
Bloom CM
影响因子：
8.2
作者：
Choleris, E;Thomas, AW;Prato, FS
通讯作者：
Prato, FS
DOI：
10.1016/j.jaac.2010.05.017
发表时间：
2010-10
影响因子：
13.3
作者：
Merikangas KR;He JP;Burstein M;Swanson SA;Avenevoli S;Cui L;Benjet C;Georgiades K;Swendsen J
通讯作者：
Swendsen J