Quantifying the Relationship between SARS-CoV-2 Wastewater Concentrations and Building-Level COVID-19 Prevalence at an Isolation Residence: A Passive Sampling Approach.
Quantifying the Relationship between SARS-CoV-2 Wastewater Concentrations and Building-Level COVID-19 Prevalence at an Isolation Residence: A Passive Sampling Approach.
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DOI：
10.3390/ijerph191811245
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发表时间：
2022-09-07
影响因子：
--
通讯作者：
Butler, Caitlyn S.
中科院分区：
文献类型：
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作者：
Acer, Patrick T.;Kelly, Lauren M.;Lover, Andrew A.;Butler, Caitlyn S.
关键词：
SARS-CoV-2 RNA 载量可在 COVID-19 患者的排泄物中检测到，并已证明与临床感染趋势呈正相关。因此，基于废水的流行病学（WBE）方法已作为公共卫生监测工具在全球范围内实施，以监测社区一级的感染流行情况。大多数废水样本通过自动采样器（自动采样器）采集为复合样本或抓取样本。然而，自动采样器价格昂贵，并且在寒冷天气下维护起来可能具有挑战性，而直接从住宅楼外的复杂基质中采样污水时，抓取样本特别容易受到时间变化的影响。被动采样可以提供经济实惠、实用且可扩展的采样系统，同时保持可重复的 SARS-CoV-2 信号。为此，我们于 2021 年 2 月 1 日至 2021 年 5 月 21 日期间在大学校园的 COVID-19 隔离单元（隔离宿舍）外部署了卫生棉条作为被动采样器。样本 (n = 64) 每周收集 3-5 次，并在下水道中保留的平均持续时间为 24 小时。使用针对 N1 和 N2 基因片段的逆转录定量聚合酶链反应 (RT-qPCR) 对 SARS-CoV-2 RNA 进行定量。我们量化了每个人捕获的平均病毒载量以及每日病毒载量与总人数之间的关联，使用多变量模型调整协变量以提供病毒排出的基线估计。样品通过校园内两个不同的实验室管道进行处理，产生高度相关的氮气浓度。这里获得的数据强调了利用卫生棉条被动采样捕获来自 COVID-19 隔离住宅的废水中的 SARS-CoV-2 的成功，表明这种方法可以帮助为建筑物一级的公共卫生应对措施提供信息。
SARS-CoV-2 RNA loads can be detected in the excreta of individuals with COVID-19 and have demonstrated positive correlations with clinical infection trends. Consequently, wastewater-based epidemiology (WBE) approaches have been implemented globally as a public health surveillance tool to monitor community-level prevalence of infections. The majority of wastewater specimens are gathered as either composite samples via automatic samplers (autosamplers) or grab samples. However, autosamplers are expensive and can be challenging to maintain in cold weather, while grab samples are particularly susceptible to temporal variation when sampling sewage directly from complex matrices outside residential buildings. Passive sampling can provide an affordable, practical, and scalable sampling system while maintaining a reproducible SARS-CoV-2 signal. In this regard, we deployed tampons as passive samplers outside of a COVID-19 isolation unit (a segregated residence hall) at a university campus from 1 February 2021–21 May 2021. Samples (n = 64) were collected 3–5 times weekly and remained within the sewer for a median duration of 24 h. SARS-CoV-2 RNA was quantified using reverse-transcription quantitative polymerase chain reaction (RT-qPCR) targeting the N1 and N2 gene fragments. We quantified the mean viral load captured per individual and the association between the daily viral load and total persons, adjusting for covariates using multivariable models to provide a baseline estimate of viral shedding. Samples were processed through two distinct laboratory pipelines on campus, yielding highly correlated N2 concentrations. Data obtained here highlight the success of passive sampling utilizing tampons to capture SARS-CoV-2 in wastewater coming from a COVID-19 isolation residence, indicating that this method can help inform building-level public health responses.
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影响因子：
3.7
作者：
Castro-Gutierrez V;Hassard F;Vu M;Leitao R;Burczynska B;Wildeboer D;Stanton I;Rahimzadeh S;Baio G;Garelick H;Hofman J;Kasprzyk-Hordern B;Kwiatkowska R;Majeed A;Priest S;Grimsley J;Lundy L;Singer AC;Di Cesare M
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Karthikeyan S;Nguyen A;McDonald D;Zong Y;Ronquillo N;Ren J;Zou J;Farmer S;Humphrey G;Henderson D;Javidi T;Messer K;Anderson C;Schooley R;Martin NK;Knight R
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影响因子：
11.4
作者：
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通讯作者：
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影响因子：
6.4
作者：
Karthikeyan S;Ronquillo N;Belda-Ferre P;Alvarado D;Javidi T;Longhurst CA;Knight R
通讯作者：
Knight R
DOI：
10.1016/j.scitotenv.2021.146749
发表时间：
2021-08-15
期刊：
The Science of the total environment
影响因子：
--
作者：
Gibas C;Lambirth K;Mittal N;Juel MAI;Barua VB;Roppolo Brazell L;Hinton K;Lontai J;Stark N;Young I;Quach C;Russ M;Kauer J;Nicolosi B;Chen D;Akella S;Tang W;Schlueter J;Munir M
通讯作者：
Munir M