Life-extending dietary restriction and ovariectomy result in similar feeding rates but different physiologic responses in grasshoppers.
Life-extending dietary restriction and ovariectomy result in similar feeding rates but different physiologic responses in grasshoppers.
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DOI：
10.1016/j.exger.2011.06.003
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发表时间：
2011-10
影响因子：
3.9
通讯作者：
Hatle, J. D.
中科院分区：
文献类型：
--
作者：
Drewry, M. D.;Williams, J. M.;Hatle, J. D.
饮食限制（DR）和繁殖减少都会延长许多物种的寿命。接受 DR 的女性通常会出现生育能力下降的情况，这引发了一个问题：这两种治疗方法是否实际上以重叠的方式延长了寿命。已证明 DR 和卵巢切除术 (OVX) 可以延长蚱蜢的寿命。在这里，我们测试这些组合对寿命的影响。如果两种治疗方法延长的寿命是相加的，则表明它们可能通过不同的途径发挥作用。实验组为：完全繁殖和完全喂养（ShamFD）；卵巢切除且完全喂养（OVX FD）；完全生殖和限制饮食（ShamDR）；以及切除卵巢和限制饮食（OVX DR）。这些组的中位寿命为：ShamFD = 245 d，OVX FD = 285 d，ShamDR = 286 d，OVX DR = 322 d。 OVX FD 组的饲喂率为随意采食的 64%，与 ShamDR 所用的随意采食的 70% 没有显着差异。我们还测量了这些个体的血淋巴生理参数。 OVX 使血淋巴中卵黄蛋白原（蛋黄前体蛋白）水平增加 5 倍，但不受 DR 影响。此外，OVX显着降低了血淋巴总抗氧化活性（每μg蛋白质），但不受DR影响。我们发现，OVX 和 DR 在蚱蜢中产生不同的生理反应，尽管寿命延长和摄食水平没有显着差异。这些数据表明 OVX 和 DR 可能通过不同的途径延长寿命。
Dietary restriction (DR) and reduced reproduction each extend life span in many species. Females undergoing DR typically experience a reduction in their fecundity, which raises the question of whether the two treatments are actually extending life span in overlapping ways. Life span in lubber grasshoppers has been shown to be increased by DR, and separately by ovariectomy (OVX). Here, we test the combination of these on life span. If life extension by the two treatments are additive, it would suggest that they likely act through separate pathways. The experimental groups were: fully reproductive and fully fed (ShamFD); ovariectomized and fully fed (OVX FD); fully reproductive and restricted diet (ShamDR); and ovariectomized and restricted diet (OVX DR). The median life spans of these groups were: ShamFD = 245 d, OVX FD = 285 d, ShamDR = 286 d, and OVX DR = 322 d. Feeding rate for the OVX FD group was 64% of ad libitum, not significantly different from the 70% of ad libitum that was used for ShamDR. We also measured hemolymph parameters of physiology in these same individuals. Hemolymph levels of vitellogenin (the egg yolk-precursor protein) were increased 5-fold by OVX, but were not affected by DR. In addition, hemolymph total anti-oxidant activity (per μg protein) was significantly reduced by OVX, but was not affected by DR. We show that OVX and DR produce different physiological responses in grasshoppers, despite life extensions and feeding levels that were not significantly different. These data suggest that OVX and DR might extend life span via distinct pathways.
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