Genome-Wide Identification and Characterization of Growth Regulatory Factor Family Genes in Medicago.
Genome-Wide Identification and Characterization of Growth Regulatory Factor Family Genes in Medicago.
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苜蓿生长调节因子家族基因的全基因组鉴定和表征
DOI：
10.3390/ijms23136905
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发表时间：
2022-06-21
影响因子：
5.6
通讯作者：
Pang, Yongzhen
中科院分区：
文献类型：
--
作者：
Du, Wenxuan;Yang, Junfeng;Li, Qian;Su, Qian;Yi, Dengxia;Pang, Yongzhen
生长调节因子（Growth Regulatory Factors，GRF）是一类植物特异性转录因子，在植物的生长发育和抗逆性中起重要作用。本研究共从蒺藜苜蓿和紫花苜蓿基因组中鉴定出16个GRF基因。多序列比对分析表明，所有这些成员都含有保守的QLQ和WRC结构域。系统进化分析表明，这些GRF蛋白可分为5个簇。GRF基因显示出相似的外显子-内含子结构和相似的保守基序结构。在它们的启动子区域发现了许多与胁迫相关的顺式作用元件，其中大部分与干旱和防御反应有关。此外，微阵列和转录组数据的分析表明，这些GRF基因表现出不同的表达模式，在不同的组织或响应干旱和盐处理。特别是，qPCR结果表明，MtGRF2-MsGRF2和MtGRF6-MsGRF6基因对的表达水平在NaCl和甘露醇处理下显著增加，表明它们最有可能参与盐和干旱胁迫的耐受。本研究为进一步研究苜蓿GRF基因的功能以及探索GRF基因在高抗苜蓿分子育种中的应用提供了理论依据。sativa.
Growth Regulatory Factors (GRF) are plant-specific transcription factors that play critical roles in plant growth and development as well as plant tolerance against stress. In this study, a total of 16 GRF genes were identified from the genomes of Medicago truncatula and Medicago sativa. Multiple sequence alignment analysis showed that all these members contain conserved QLQ and WRC domains. Phylogenetic analysis suggested that these GRF proteins could be classified into five clusters. The GRF genes showed similar exon–intron organizations and similar architectures in their conserved motifs. Many stress-related cis-acting elements were found in their promoter region, and most of them were related to drought and defense response. In addition, analyses on microarray and transcriptome data indicated that these GRF genes exhibited distinct expression patterns in various tissues or in response to drought and salt treatments. In particular, qPCR results showed that the expression levels of gene pairs MtGRF2–MsGRF2 and MtGRF6–MsGRF6 were significantly increased under NaCl and mannitol treatments, indicating that they are most likely involved in salt and drought stress tolerance. Collectively, our study is valuable for further investigation on the function of GRF genes in Medicago and for the exploration of GRF genes in the molecular breeding of highly resistant M. sativa.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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