Maternal origins of developmental reproducibility.
Maternal origins of developmental reproducibility.
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DOI：
10.1016/j.cub.2014.04.028
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发表时间：
2014-06-02
期刊：
影响因子：
9.2
通讯作者：
Gregor, Thomas
中科院分区：
文献类型：
--
作者：
Petkova, Mariela D.;Little, Shawn C.;Liu, Feng;Gregor, Thomas
多细胞发育过程中的细胞命运决定是精确协调的，导致了高度可重复性的宏观结构结果。这种重复性的起源是在发育的最早阶段的分子水平上发现的，当时形态分子的模式是可复制的。然而，虽然这些早期阶段的初始条件是由雌性在卵子发生期间决定的，但尚不清楚繁殖力是从卵子发生后永久存在还是由受精卵重新获得。为了解决这个问题，在果蝇早期胚胎中，我们试图计算单个母体沉积的双核mRNA分子，并将胚胎之间的变异性与先前观察到的双核蛋白梯度的波动进行比较。在这里，我们开发了独立的方法来量化单个胚胎中的mRNA总量，并表明胚胎之间的mRNA计数的重复性很高，在~9%以内，与蛋白质梯度的重复性相匹配。重复性来自完美的线性前馈过程：改变雌性的遗传剂量会导致胚胎中的mRNA和蛋白质数量成比例的变化。我们的结果表明，胚胎形态结构的重复性起源于卵子发生期间，当时最初的模式信号得到了精确的控制。
Cell fate decisions during multicellular development are precisely coordinated, leading to highly reproducible macroscopic structural outcomes. The origins of this reproducibility are found at the molecular level during the earliest stages of development, when patterns of morphogen molecules emerge reproducibly. However, while the initial conditions for these early stages are determined by the female during oogenesis, it is unknown whether reproducibility is perpetuated from oogenesis or reacquired by the zygote. To address this issue in the early Drosophila embryo, we sought to count individual maternally deposited bicoid mRNA molecules and compare variability between embryos with previously observed fluctuations in the Bicoid protein gradient. Here we develop independent methods to quantify total amounts of mRNA in individual embryos and show that mRNA counts are highly reproducible between embryos to within ~9%, matching the reproducibility of the protein gradient. Reproducibility emerges from perfectly linear feed-forward processes: changing the genetic dosage in the female leads to proportional changes in the mRNA and protein numbers in the embryo. Our results indicate that the reproducibility of the morphological structures of embryos originates during oogenesis when initial patterning signals are precisely controlled.
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影响因子：
9.8
作者：
Little SC;Tkačik G;Kneeland TB;Wieschaus EF;Gregor T
通讯作者：
Gregor T
影响因子：
8.6
作者：
Erdmann, Thorsten;Howard, Martin;ten Wolde, Pieter Rein
通讯作者：
ten Wolde, Pieter Rein
影响因子：
64.5
作者：
Little SC;Tikhonov M;Gregor T
通讯作者：
Gregor T
影响因子：
64.5
作者：
DRIEVER, W;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C
DOI：
10.1007/bf00337800
发表时间：
1979-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
BONVEN, B;GULLOV, K
通讯作者：
GULLOV, K