Cytological, molecular mechanisms and temperature stress regulating production of diploid male gametes in Dianthus caryophyllus L.
Cytological, molecular mechanisms and temperature stress regulating production of diploid male gametes in Dianthus caryophyllus L.
复制标题
石竹二倍体雄配子产生的细胞学、分子机制和温度胁迫调节。
DOI：
10.1016/j.plaphy.2015.10.003
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发表时间：
2015-12
影响因子：
6.5
通讯作者：
Wenru Tang
中科院分区：
文献类型：
--
作者：
Xuhong Zhou;Xijun Mo;Min Gui;Xuewei Wu;Yalian Jiang;Lulin Ma;Ziming Shi;Ying Luo;Wenru Tang
在植物进化中，由于其在有性多倍化或全基因组复制事件中的关键作用，二倍体配子形成被认为是多样化和物种形成的重要组成部分。环境压力常常会引发配子产生的不减少。然而，人们对康乃馨双配子产生的分子、细胞机制和不利的温度调节仍知之甚少。在这里，我们研究了 2n 雄配子形成的细胞学基础，并描述了第一个基因 DcPS1（石竹平行纺锤体 1）的分离和表征。此外，我们分析了温度胁迫对二倍体配子形成和DcPS1转录水平的影响。细胞学证据表明，2n 雄配子的形成可归因于雄性减数分裂 II 时纺锤体方向异常。DcPS1 蛋白在整个植物界中是保守的，并携带暗示调节功能的结构域。DcPS1 表达分析表明，DcPS1 基因可能在 2n 花粉形成中发挥作用。各种栽培中未减少的花粉形成对高温或低温敏感，这可能是受DcPS1转录本水平调节的。从更广泛的角度来看，这些发现可以在基础多倍体研究和植物育种计划中具有潜在的应用。
In plant evolution, because of its key role in sexual polyploidization or whole genome duplication events, diploid gamete formation is considered as an important component in diversification and speciation. Environmental stress often triggers unreduced gamete production. However, the molecular, cellular mechanisms and adverse temperature regulating diplogamete production in carnation remain poorly understood. Here, we investigate the cytological basis for 2n male gamete formation and describe the isolation and characterization of the first gene,DcPS1(Dianthus Caryophyllus Parallel Spindle 1). In addition, we analyze influence of temperature stress on diploid gamete formation and transcript levels ofDcPS1. Cytological evidence indicated that 2n male gamete formation is attributable to abnormal spindle orientation at male meiosis II.DcPS1protein is conserved throughout the plant kingdom and carries domains suggestive of a regulatory function.DcPS1expression analysis showDcPS1gene probably have a role in 2n pollen formation. Unreduced pollen formation in various cultivation was sensitive to high or low temperature which was probably regulated by the level ofDcPS1transcripts. In a broader perspective, these findings can have potential applications in fundamental polyploidization research and plant breeding programs.
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影响因子：
6.9
作者：
Pecrix, Yann;Rallo, Geraldine;Le Bris, Manuel
通讯作者：
Le Bris, Manuel
影响因子：
1.9
作者：
R. Ssebuliba;A. Tenkouano;M. Pillay
通讯作者：
R. Ssebuliba;A. Tenkouano;M. Pillay
影响因子：
3.1
作者：
G. Genualdo;A. Errico;A. Tiezzi;C. Conicella
通讯作者：
G. Genualdo;A. Errico;A. Tiezzi;C. Conicella
影响因子：
1.9
作者：
Xuhong Zhou;M. Gui;Da-yao Zhao;Min Chen;Suping Ju;She-song Li;Zhe Lu;Xijun Mo;Jihua Wang-
通讯作者：
Xuhong Zhou;M. Gui;Da-yao Zhao;Min Chen;Suping Ju;She-song Li;Zhe Lu;Xijun Mo;Jihua Wang-
影响因子：
1.9
作者：
Crespel, L.;Ricci, S. C.;Gudin, S.
通讯作者：
Gudin, S.