Common themes and cell type specific variations of higher order chromatin arrangements in the mouse.
Common themes and cell type specific variations of higher order chromatin arrangements in the mouse.
复制标题
DOI：
10.1186/1471-2121-6-44
复制
发表时间：
2005-12-07
期刊：
影响因子：
--
通讯作者：
Dietzel S
中科院分区：
文献类型：
--
作者：
Mayer R;Brero A;von Hase J;Schroeder T;Cremer T;Dietzel S
人类细胞类型的高阶染色质排列的相似性和差异性先前已有报道。为了进行进化比较，我们用荧光原位杂交和共聚焦激光扫描显微镜研究了六种小鼠细胞类型（淋巴细胞、胚胎干细胞、巨噬细胞、成纤维细胞、成肌细胞和肌管）的染色体区域和着丝粒区域的排列。这两个物种进化显着的核型差异后，他们最后的共同祖先生活在约8700万年前，因此似乎特别适合阐明共同的和细胞类型的特定主题的高阶染色质安排在哺乳动物。所有小鼠细胞类型的径向染色体领土的位置与基因密度以及与染色体大小的非随机相关性。在分化过程中，染色体区域和着丝粒周围异染色质的分布发生变化，导致不同的细胞类型特异性分布模式。我们排除了这些差异对核形状的严格依赖。小鼠细胞核中的位置差异与人类细胞核相比不太明显，这与染色体大小和基因密度的较小差异一致。值得注意的是，染色体区域相对于彼此的位置非常可变。根据染色体大小和基因密度的染色体区域排列在人和小鼠细胞类型中提供了共同的、进化上保守的主题。我们的研究结果与先前报道的亲本基因组分离模型不一致。
Similarities as well as differences in higher order chromatin arrangements of human cell types were previously reported. For an evolutionary comparison, we now studied the arrangements of chromosome territories and centromere regions in six mouse cell types (lymphocytes, embryonic stem cells, macrophages, fibroblasts, myoblasts and myotubes) with fluorescence in situ hybridization and confocal laser scanning microscopy. Both species evolved pronounced differences in karyotypes after their last common ancestors lived about 87 million years ago and thus seem particularly suited to elucidate common and cell type specific themes of higher order chromatin arrangements in mammals. All mouse cell types showed non-random correlations of radial chromosome territory positions with gene density as well as with chromosome size. The distribution of chromosome territories and pericentromeric heterochromatin changed during differentiation, leading to distinct cell type specific distribution patterns. We exclude a strict dependence of these differences on nuclear shape. Positional differences in mouse cell nuclei were less pronounced compared to human cell nuclei in agreement with smaller differences in chromosome size and gene density. Notably, the position of chromosome territories relative to each other was very variable. Chromosome territory arrangements according to chromosome size and gene density provide common, evolutionary conserved themes in both, human and mouse cell types. Our findings are incompatible with a previously reported model of parental genome separation.
登录
查看更多内容
DOI：
10.1083/jcb.145.6.1119
发表时间：
1999-06-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Croft JA;Bridger JM;Boyle S;Perry P;Teague P;Bickmore WA
通讯作者：
Bickmore WA
影响因子：
21.3
作者：
Brown, KE;Amoils, S;Fisher, AG
通讯作者：
Fisher, AG
影响因子：
20.3
作者：
Alcobia, I;Dilao, R;Parreira, L
通讯作者：
Parreira, L
影响因子：
3.4
作者：
Bin Sun, H;Shen, J;Yokota, H
通讯作者：
Yokota, H
影响因子：
7.8
作者：
Cornforth, MN;Greulich-Bode, KM;Brenner, DJ
通讯作者：
Brenner, DJ