Assessment of creatinine concentration in whole blood spheroids using paper spray ionization-tandem mass spectrometry.
Assessment of creatinine concentration in whole blood spheroids using paper spray ionization-tandem mass spectrometry.
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DOI：
10.1038/s41598-022-18365-8
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发表时间：
2022-08-22
影响因子：
4.6
通讯作者：
Maher, Simon
中科院分区：
文献类型：
--
作者：
Sham, Tung-Ting;Badu-Tawiah, Abraham K.;McWilliam, Stephen J.;Maher, Simon
准确定量血肌酐对于估计肾小球滤过率很重要。使用液相色谱串联质谱 (LC-MS/MS) 的现有技术具有高精度，并消除了常规酶法和 Jaffé 方法中遇到的大多数干扰。然而，它们需要费力且耗时的样品处理和数据采集。本研究的目的是开发一种快速、简单的方法，能够直接分析全血肌酐，其性能测量与传统 LC-MS/MS 相当。 5μL 全血在疏水性硅烷化纸基质上形成三维球体，然后进行纸喷雾电离串联质谱 (PSI-MS/MS)。该方法使用真实人体样本进行验证，并与 LC-MS/MS 进行比较。 PSI–MS/MS 全血分析的定量下限为 2.5 µg/mL，精度≤≤6.3%，回收率在 88–94% 范围内，线性度极佳（R2 > 0.99；2.5–20 µg/mL），涵盖肌酐水平的正常范围。肌酐水平与 LC-MS/MS 测量的水平相当，偏差小于 0.3 µg/mL。这种简单、快速且准确的微量采样技术可直接分析全血中的肌酐，为常规临床筛查和监测带来了希望。这种方法可以很容易地扩展到其他感兴趣的分析物，并且由于与成本、可存储性、速度和简单性相关的固有优势，它对于在资源有限的环境中使用尤其有利。
Accurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC–MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). The method is validated using real human samples and compared with LC–MS/MS. PSI–MS/MS whole blood analysis exhibited a lower limit of quantification of 2.5 μg/mL, precision ≤ 6.3%, recovery in the range of 88–94% and excellent linearity (R2 > 0.99; 2.5—20 μg/mL) covering the normal range for creatinine levels. Creatinine levels were comparable to those measured by LC–MS/MS with small deviations of less than 0.3 μg/mL. This simple, fast and accurate microsampling technique for direct analysis of creatinine from whole blood shows promise for routine clinical screening and monitoring. This approach can be readily extended for other analytes of interest and, due to inherent advantages relating to cost, storability, speed, and simplicity, it can be especially advantageous for use in resource-limited settings.
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影响因子：
2.9
作者：
Mian AN;Schwartz GJ
通讯作者：
Schwartz GJ
影响因子：
4.6
作者：
Sarih NM;Romero-Perez D;Bastani B;Rauytanapanit M;Boisdon C;Praneenararat T;Tajuddin HA;Abdullah Z;Badu-Tawiah AK;Maher S
通讯作者：
Maher S
干血球的保护机制：稳定全血、血浆和血清中的不稳定分析物。
DOI：
10.1039/d1an01132d
发表时间：
2021-11-08
期刊：
The Analyst
影响因子：
--
作者：
Frey BS;Damon DE;Allen DM;Baker J;Asamoah S;Badu-Tawiah AK
通讯作者：
Badu-Tawiah AK
影响因子：
2.1
作者：
Bernstone, Laura;Jayanti, Anuradha;Keevil, Brian
通讯作者：
Keevil, Brian
影响因子：
9.4
作者：
Murphy, Sean C.;Daza, Glenda;Coombs, Robert
通讯作者：
Coombs, Robert