Protective mechanism of dried blood spheroids: stabilization of labile analytes in whole blood, plasma, and serum.
Protective mechanism of dried blood spheroids: stabilization of labile analytes in whole blood, plasma, and serum.
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干血球的保护机制：稳定全血、血浆和血清中的不稳定分析物。
DOI：
10.1039/d1an01132d
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发表时间：
2021-11-08
期刊：
影响因子：
--
通讯作者：
Badu-Tawiah AK
中科院分区：
文献类型：
--
作者：
Frey BS;Damon DE;Allen DM;Baker J;Asamoah S;Badu-Tawiah AK
当全血沉积在疏水性纸上并在周围空气中干燥时，就会形成三维（3D）干血球体。研究人员观察到，疏水性纸表面吸附的3D干血球体为不稳定分析物提供了更高的稳定性，否则，如果用传统的二维（2D）干血点法储存，不稳定分析物会降解。利用扫描电子显微镜（SEM）研究了干燥血液球体微采样平台的保护机制，发现在球体表面存在钝化薄膜，用于稳定球体内部免受环境胁迫。通过基于序列扫描电镜分析的时间过程实验，我们发现，随着血液样本中水分的蒸发，红细胞的自组装形成了表面保护薄膜。在我们的实验中，红细胞聚集的桥接机制是显而易见的，这导致在形成血球后不到60分钟内堆积的红细胞形成明显的rouleau构象。球体外部的自组装红细胞堆叠随后溶解，在环境空气中储存三周后，检测到的表面保护层厚度约为30微米。我们将这种机制应用于血浆和血清，以提高在环境条件下储存时的稳定性。除了对这些薄生物膜进行物理表征外，我们还使用了纸喷雾质谱（MS）来检查储存的生物流体中发生的化学变化。例如，我们提供了全血、血浆和血清中可卡因在环境条件下在亲水性和疏水性纸基质上储存时的稳定性数据。
Three-dimensional (3D) dried blood spheroids form when whole blood is deposited onto hydrophobic paper and allowed to dry in ambient air. The adsorbed 3D dried blood spheroid present at the surface of the hydrophobic paper is observed to offer enhanced stability for labile analytes that would otherwise degrade if stored in the traditional two-dimensional (2D) dried blood spot method. The protective mechanism for the dried blood spheroid microsampling platform was studied using scanning electron microscopy (SEM), which revealed the presence of a passivation thin film at the surface of the spheroid that serves to stabilize the interior of the spheroid against environmental stressors. Through time-course experiments based on sequential SEM analyses, we discovered that the surface protective thin film forms through the self-assembly of red blood cells following the evaporation of water from the blood sample. The bridging mechanism of red blood cell aggregation is evident in our experiments, which leads to the distinct rouleau conformation of stacked red blood cells in less than 60 min after creating the blood spheroid. The stack of self-assembled red blood cells at the exterior of the spheroid subsequently lyse to afford the surface protective layer detected to be approximately 30 µm in thickness after three weeks of storage in ambient air. We applied this mechanistic insight to plasma and serum to enhance stability when stored under ambient conditions. In addition to physical characterization of these thin biofilms, we also used paper spray mass spectrometry (MS) to examine chemical changes that occur in the stored biofluid. For example, we present stability data for cocaine spiked in whole blood, plasma, and serum when stored under ambient conditions on hydrophilic and hydrophobic paper substrates.
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影响因子：
5
作者：
Riegman, P. H. J.;Dinjens, W. N. M.;Oosterhuis, J. W.
通讯作者：
Oosterhuis, J. W.
影响因子：
10.4
作者：
Olshan, Andrew F.
通讯作者：
Olshan, Andrew F.
影响因子：
7.4
作者：
Damon, Deidre E.;Yin, Mengzhen;Badu-Tawiah, Abraham K.
通讯作者：
Badu-Tawiah, Abraham K.
影响因子：
15.6
作者：
Sefiane, Khellil
通讯作者：
Sefiane, Khellil
DOI：
10.1016/0035-9203(60)90086-9
发表时间：
1960-01-01
期刊：
TRANS ROY SOC TROP MED AND HYG
影响因子：
--
作者：
SHUTE, P. G.;MARYON, M.
通讯作者：
MARYON, M.