Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
Fabrication of Antimicrobial Peptide-Loaded PLGA/Chitosan Composite Microspheres for Long-Acting Bacterial Resistance.
复制标题
长效抗菌肽负载PLGA/壳聚糖复合微球的制备
DOI：
10.3390/molecules22101637
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发表时间：
2017-09-29
期刊：
影响因子：
--
通讯作者：
Cui F
中科院分区：
文献类型：
--
作者：
Li Y;Na R;Wang X;Liu H;Zhao L;Sun X;Ma G;Cui F
抗菌十肽KSL-W(KKVVFWVKFK-CONH2)可以在唾液中保持稳定的抗菌活性，因此被广泛用于抑制牙齿上生物膜的形成和防止口腔微生物的生长，用于相关感染性疾病的治疗。为了控制KSL-W对细菌的长期耐药性，采用电喷雾和复合交联法制备了KSL-W PLGA/壳聚糖复合微球(KSL/PLGA/CS MSS)。对不同剂型的微球进行了表面形态、粒径分布、包封率、体外释药和抗菌活性等方面的表征。抑菌实验表明，KSL/PLGA/CS MSS对口腔细菌具有持久的抑菌和抑菌作用。此外，细胞增殖实验证明，释放的KSL-W抗菌剂量对成骨细胞MC3T3-E1的生长无细胞毒性。因此，我们的研究表明，KSL-W-PLGA/CS复合微球在治疗牙周炎和牙周炎等口腔感染性疾病以及骨移植替代牙槽骨增强方面可能具有潜在的治疗应用前景。
An antimicrobial decapeptide, KSL-W (KKVVFWVKFK-CONH2), which could maintain stable antimicrobial activity in saliva, has therefore been widely used to inhibit biofilm formation on teeth and prevent the growth of oral microorganisms for related infectious diseases treatment. In order to control the release of KSL-W for long-term bacterial resistance, KSL-W-loaded PLGA/chitosan composite microspheres (KSL/PLGA/CS MSs) were prepared by electrospraying and combined crosslinking-emulsion methods. Different formulations of microspheres were characterized as to surface morphology, size distribution, encapsulation efficiency, in vitro drug release, and antimicrobial activity. Antibacterial experiment demonstrated the prolonged antimicrobial and inhibitory effects of KSL/PLGA/CS MSs on oral bacteria. Moreover, the cell proliferation assay proved that the released KSL-W antibacterial dosage had no cytotoxicity to the growth of osteoblast MC3T3-E1. Thus, our study suggested that the KSL-W-loaded PLGA/CS composite microspheres may have potentially therapeutic applications as an effective drug delivery system in the treatment of oral infectious diseases such as periodontitis and periodontitis, and also within bone graft substitutes for alveolar bone augmentation.
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影响因子：
6.7
作者：
Yao S;Liu H;Yu S;Li Y;Wang X;Wang L
通讯作者：
Wang L
DOI：
10.2217/nnm.11.92
发表时间：
2011-09
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Hood E;Simone E;Wattamwar P;Dziubla T;Muzykantov V
通讯作者：
Muzykantov V
影响因子：
11.2
作者：
Dev, Ashish;Binulal, N. S.;Jayakumar, R.
通讯作者：
Jayakumar, R.
影响因子：
7.6
作者：
Leung, KP;Crowe, TD;Thompson, GA
通讯作者：
Thompson, GA
DOI：
10.1016/j.ejpb.2005.11.003
发表时间：
2006-05-01
影响因子：
4.9
作者：
Sebti, T;Amighi, K
通讯作者：
Amighi, K